Acidic pH or salt treatment can convert soluble antibody aggregates in culture harvest into monomers and improve Protein A chromatography step yield.

Zhang, Ting; Li, Bin; Dong, Wanyuan; Wan, Yan; Li, Yifeng

Zhang, Ting; Li, Bin; Dong, Wanyuan; Wan, Yan; Li, Yifeng.

Afiliación

Zhang T; Downstream Process Development (DSPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China.
Li B; Downstream Process Development (DSPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China.
Dong W; Downstream Process Development (DSPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China.
Wan Y; Downstream Process Development (DSPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China. Electronic address: wan_yan_010657@wuxibiologics.com.
Li Y; Downstream Process Development (DSPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China. Electronic address: li_yifeng@wuxibiologics.com.

Protein Expr Purif ; 215: 106391, 2024 Mar.

Article en En | MEDLINE | ID: mdl-37939750

Asunto(s)

Anticuerpos Monoclonales; Proteína Estafilocócica A; Anticuerpos Monoclonales/química; Proteína Estafilocócica A/química; Cromatografía; Concentración de Iones de Hidrógeno

Palabras clave

Acidic pH; Aggregate; Antibody; Monomer; Protein A chromatography; Salt

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteína Estafilocócica A / Anticuerpos Monoclonales Idioma: En Revista: Protein Expr Purif Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos

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