In vitro selection of aptamers and their applications.

DeRosa, M C; Lin, A; Mallikaratchy, P; McConnell, E M; McKeague, M; Patel, R; Shigdar, S

DeRosa, M C; Lin, A; Mallikaratchy, P; McConnell, E M; McKeague, M; Patel, R; Shigdar, S.

Afiliación

DeRosa MC; Department of Chemistry and Institute of Biochemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON, Canada K1T2S2.
Lin A; Department of Chemistry, Faculty of Sciences, McGill University, Montreal, QC, Canada, H3A 0B8.
Mallikaratchy P; Department of Molecular, Cellular, and Biomedical Sciences, City University of New York School of Medicine, New York, NY 10031, USA.
McConnell EM; Ph.D. Programs in Chemistry and Biochemistry, CUNY Graduate Center, 365 Fifth Avenue, New York, NY 10016, USA.
McKeague M; Ph.D. Program in Molecular, Cellular and Developmental Biology, CUNY Graduate Center, 365 Fifth Avenue, New York, NY 10016, USA.
Patel R; Department of Chemistry and Institute of Biochemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON, Canada K1T2S2.
Shigdar S; Department of Chemistry, Faculty of Sciences, McGill University, Montreal, QC, Canada, H3A 0B8.

Nat Rev Methods Primers ; 32023.

Article en En | MEDLINE | ID: mdl-37969927

ABSTRACT

ABSTRACT

The introduction of the in-vitro evolution method known as SELEX (Systematic Evolution of Ligands by Exponential enrichment) more than 30 years ago led to the conception of versatile synthetic receptors known as aptamers. Offering many benefits such as low cost, high stability and flexibility, aptamers have sparked innovation in molecular diagnostics, enabled advances in synthetic biology and have facilitated new therapeutic approaches. The SELEX method itself is inherently adaptable and offers near limitless possibilities in yielding functional nucleic acid ligands. This Primer serves to provide guidance on experimental design and highlight new growth areas for this impactful technology.

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Nat Rev Methods Primers Año: 2023 Tipo del documento: Article