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Reduced FOXF1 links unrepaired DNA damage to pulmonary arterial hypertension.
Isobe, Sarasa; Nair, Ramesh V; Kang, Helen Y; Wang, Lingli; Moonen, Jan-Renier; Shinohara, Tsutomu; Cao, Aiqin; Taylor, Shalina; Otsuki, Shoichiro; Marciano, David P; Harper, Rebecca L; Adil, Mir S; Zhang, Chongyang; Lago-Docampo, Mauro; Körbelin, Jakob; Engreitz, Jesse M; Snyder, Michael P; Rabinovitch, Marlene.
Afiliación
  • Isobe S; Basic Science and Engineering (BASE) Initiative at the Betty Irene Moore Children's Heart Center, Lucile Packard Children's Hospital, Stanford University School of Medicine, Stanford, CA, USA.
  • Nair RV; Vera Moulton Wall Center for Pulmonary Vascular Diseases, Stanford University, Stanford, CA, USA.
  • Kang HY; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA.
  • Wang L; Department of Pediatrics - Cardiology, Stanford University School of Medicine, Stanford, CA, USA.
  • Moonen JR; Stanford Center for Genomics and Personalized Medicine, Stanford University School of Medicine, Stanford, CA, USA.
  • Shinohara T; Basic Science and Engineering (BASE) Initiative at the Betty Irene Moore Children's Heart Center, Lucile Packard Children's Hospital, Stanford University School of Medicine, Stanford, CA, USA.
  • Cao A; Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.
  • Taylor S; Basic Science and Engineering (BASE) Initiative at the Betty Irene Moore Children's Heart Center, Lucile Packard Children's Hospital, Stanford University School of Medicine, Stanford, CA, USA.
  • Otsuki S; Vera Moulton Wall Center for Pulmonary Vascular Diseases, Stanford University, Stanford, CA, USA.
  • Marciano DP; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA.
  • Harper RL; Department of Pediatrics - Cardiology, Stanford University School of Medicine, Stanford, CA, USA.
  • Adil MS; Basic Science and Engineering (BASE) Initiative at the Betty Irene Moore Children's Heart Center, Lucile Packard Children's Hospital, Stanford University School of Medicine, Stanford, CA, USA.
  • Zhang C; Vera Moulton Wall Center for Pulmonary Vascular Diseases, Stanford University, Stanford, CA, USA.
  • Lago-Docampo M; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA.
  • Körbelin J; Department of Pediatrics - Cardiology, Stanford University School of Medicine, Stanford, CA, USA.
  • Engreitz JM; Basic Science and Engineering (BASE) Initiative at the Betty Irene Moore Children's Heart Center, Lucile Packard Children's Hospital, Stanford University School of Medicine, Stanford, CA, USA.
  • Snyder MP; Vera Moulton Wall Center for Pulmonary Vascular Diseases, Stanford University, Stanford, CA, USA.
  • Rabinovitch M; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA.
Nat Commun ; 14(1): 7578, 2023 Nov 21.
Article en En | MEDLINE | ID: mdl-37989727
ABSTRACT
Pulmonary arterial hypertension (PAH) is a progressive disease in which pulmonary arterial (PA) endothelial cell (EC) dysfunction is associated with unrepaired DNA damage. BMPR2 is the most common genetic cause of PAH. We report that human PAEC with reduced BMPR2 have persistent DNA damage in room air after hypoxia (reoxygenation), as do mice with EC-specific deletion of Bmpr2 (EC-Bmpr2-/-) and persistent pulmonary hypertension. Similar findings are observed in PAEC with loss of the DNA damage sensor ATM, and in mice with Atm deleted in EC (EC-Atm-/-). Gene expression analysis of EC-Atm-/- and EC-Bmpr2-/- lung EC reveals reduced Foxf1, a transcription factor with selectivity for lung EC. Reducing FOXF1 in control PAEC induces DNA damage and impaired angiogenesis whereas transfection of FOXF1 in PAH PAEC repairs DNA damage and restores angiogenesis. Lung EC targeted delivery of Foxf1 to reoxygenated EC-Bmpr2-/- mice repairs DNA damage, induces angiogenesis and reverses pulmonary hypertension.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hipertensión Arterial Pulmonar / Hipertensión Pulmonar Límite: Animals / Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hipertensión Arterial Pulmonar / Hipertensión Pulmonar Límite: Animals / Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos