Quest to develop a standard screening method for urothelial carcinoma using liquid-based cytology (The Paris System) and CK20.
Indian J Pathol Microbiol
; 66(4): 720-726, 2023.
Article
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| MEDLINE
| ID: mdl-38084522
ABSTRACT
Background:
Bladder cancer, the most common malignancy of the urinary tract is a leading cause of morbidity and mortality. But cystoscopy, which is till now the mainstay of screening, is an invasive, high-cost method with low sensitivity especially for flat lesions.Aim:
To find a non-invasive and effective screening method with liquid-based cytology (LBC) using The Paris System (TPS) and CK20 immunocytochemistry. Materials andMethods:
It was a prospective study including the patients with clinical or cystoscopic diagnosis of urinary bladder space occupying lesions (SOL). Both conventional (CC) and liquid-based cytology slides were prepared from urine samples. Slides were evaluated by two trained pathologists and categorized according to TPS guidelines. CK20 immunocytochemistry (ICC) was also performed. Consequent formalin-fixed paraffin embedded sections were blindly examined by another pathologist and was taken as gold standard for comparison. StatisticalAnalysis:
All the statistical analysis were done using MedCalc version 15.8 [Mariakerke, Belgium MedCalc Software 2015].Results:
The study included 150 cases with a mean age of 62.4 years. Five cases revealed unsatisfactory smears. Rest of the cases were categorized as the following 18.1% as NH-GUC, 8% as LGUN, 22.1% as AUC, 15.4% as SH-GUC, 32.9% as HGUC. Kappa value of CC and LBC were strong (0.854). LBC alone showed very low specificity (58%) and PPV (74.8%) which improved on application of ICC (specificity 97.4%, PPV 96.3%).Conclusion:
We conclude that CK20 ICC offers potential for accurate, non-invasive detection and surveillance of bladder cancer and is a better tool when combined with liquid-based cytology, reported using The Paris System.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias de la Vejiga Urinaria
/
Carcinoma de Células Transicionales
Límite:
Humans
/
Middle aged
Idioma:
En
Revista:
Indian J Pathol Microbiol
Año:
2023
Tipo del documento:
Article
País de afiliación:
India