A semi-nested PCR method with increased sensitivity for the specific, direct detection of Salmonella enterica strains in poultry ectoparasites.
Acta Vet Hung
; 71(3-4): 137-141, 2024 Jan 09.
Article
en En
| MEDLINE
| ID: mdl-38090949
ABSTRACT
The darkling beetle, Alphitobius diaperinus, and the poultry red mite, Dermanysuss gallinae are among the most common pests of poultry farms. Both pests can be carriers and reservoirs of various pathogens including zoonotic ones like Salmonella. Salmonellosis is one of the most common foodborne diseases reported in the EU. We developed a semi-nested PCR method for the direct detection of Salmonella enterica. When testing the specificity of the novel PCR, we successfully detected various S. enterica strains, whereas Escherichia coli and Citrobacter strains gave negative results. The authenticity of the PCR products was confirmed by DNA sequencing. The sensitivity of the semi-nested PCR was tested on serial dilution of bacterial cultures and extracted DNA. We found our new method more sensitive than the previous PCRs. We also screened ectoparasite samples, collected from a poultry farm in Hungary, and three out of the eight samples were positive for S. Enteritidis. This novel PCR seems suitable for the detection of S. enterica strains in poultry ectoparasites without the need of sample pre-enrichment.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de las Aves de Corral
/
Salmonella enterica
Límite:
Animals
País/Región como asunto:
Europa
Idioma:
En
Revista:
Acta Vet Hung
Año:
2024
Tipo del documento:
Article
País de afiliación:
Hungria
Pais de publicación:
HU
/
HUNGARY
/
HUNGRIA