Effects of variation in sample storage conditions and swab order on 16S vaginal microbiome analyses.
Microbiol Spectr
; 12(1): e0371223, 2024 Jan 11.
Article
en En
| MEDLINE
| ID: mdl-38095462
ABSTRACT
IMPORTANCE The composition of the human vaginal microbiome has been linked to a variety of medical conditions including yeast infection, bacterial vaginosis, and sexually transmitted infection. The vaginal microbiome is becoming increasingly acknowledged as a key factor in personal health, and it is essential to establish methods to collect and process accurate samples with self-collection techniques to allow large, population-based studies. In this study, we investigate if using AssayAssure Genelock, a nucleic acid preservative, introduces microbial biases in self-collected vaginal samples. To our knowledge, we also contribute some of the first evidence regarding the impacts of multiple swabs taken at one time point. Vaginal samples have relatively low biomass, so the ability to collect multiple swabs from a unique participant at a single time would greatly improve the replicability and data available for future studies. This will hopefully lay the groundwork to gain a more complete and accurate understanding of the vaginal microbiome.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Vagina
/
Microbiota
Límite:
Female
/
Humans
Idioma:
En
Revista:
Microbiol Spectr
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos