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An in vitro larval motility assay characterizes anthelmintic efficacy against Crenosoma vulpis, Angiostrongylus vasorum, and Aelurostrongylus abstrusus.
Mahjoub, Haifaa A; Bedenice, Daniela; Stryhn, Henrik; Yu, Jenny; Greenwood, Spencer J; Conboy, Gary A.
Afiliación
  • Mahjoub HA; Biological Sciences Department, College of Sciences & Arts, King Abdulaziz University, Rabigh 21911, Saudi Arabia.
  • Bedenice D; Department of Clinical Sciences, Tufts Cummings School of Veterinary Medicine, North Grafton, MA.
  • Stryhn H; Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada.
  • Yu J; Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada.
  • Greenwood SJ; Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada.
  • Conboy GA; Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada.
Am J Vet Res ; 85(2)2024 Feb 01.
Article en En | MEDLINE | ID: mdl-38109845
ABSTRACT

OBJECTIVE:

This study determined the in vitro efficacy of 6 common anthelmintics (eprinomectin, ivermectin, milbemycin oxime, moxidectin, selamectin, and fenbendazole) on motility (viability) of infectious third-stage larvae (L3) of Crenosoma vulpis, Angiostrongylus vasorum, and Aelurostrongylus abstrusus, which are important causes of canine and feline cardiopulmonary disease. SAMPLES First-stage larvae (L1) from C vulpis, An vasorum, and Ae abstrusus. PROCEDURES Naïve Limax maximus slugs were fed 1,000 to 2,000 L1 and held at 16 °C for at least 4 weeks to produce live L3. Approximately 50 to 100 L3/well were subsequently incubated in culture media alone or media containing 6 separate test anthelmintics at 4 concentrations, to bracket expected in vivo drug plasma levels in anthelmintic-treated dogs and cats. Drug effects on L3 motility (viability) were analyzed by multilevel logistic models, generating dose-response relationships. Experiments were completed 1-9/2019.

RESULTS:

Drug concentration estimates corresponding to a 50% larval mortality rate identified that C vulpis was the most sensitive species to the anthelmintics tested. Ae abstrusus was most susceptible to moxidectin and selamectin, while An vasorum was insusceptible to all anthelmintics tested, except for selamectin at high drug concentrations. CLINICAL RELEVANCE The in vitro anthelmintic response to antiparasitic agents may guide and improve disease therapy and prevention. Considering the observed lack of efficacy against L3, monthly anthelmintic treatment for protection against An vasorum infection in dogs would primarily rely on the anthelmintic's adulticidal activity. Maximal preventive control for An vasorum would, therefore, require at least 1 treatment administered a minimum of 1 week after the end of the transmission season.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ivermectina / Enfermedades de los Gatos / Macrólidos / Enfermedades de los Perros / Angiostrongylus / Metastrongyloidea / Antihelmínticos Límite: Animals Idioma: En Revista: Am J Vet Res Año: 2024 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ivermectina / Enfermedades de los Gatos / Macrólidos / Enfermedades de los Perros / Angiostrongylus / Metastrongyloidea / Antihelmínticos Límite: Animals Idioma: En Revista: Am J Vet Res Año: 2024 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Estados Unidos