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A time-resolved fluorescence microsphere-lateral flow immunochromatographic strip for quantitative detection of Pregnanediol-3-glucuronide in urine samples.
Lin, Jiasheng; Li, Sanhua; Ye, Benchen; Zheng, Weigang; Wang, Huihui; Liu, Ying; Wang, Dong; Wu, Zaihui; Dong, Wen-Fei; Zan, Minghui.
Afiliación
  • Lin J; CAS Key Laboratory of Biomedical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science (CAS), Suzhou, China.
  • Li S; Zhengzhou Institute of Biomedical Engineering and Technology, Zhengzhou, China.
  • Ye B; Henan Province Joint International Laboratory for Bioconjugation and Antibody Coupling, Zhengzhou, China.
  • Zheng W; Zhengzhou Institute of Biomedical Engineering and Technology, Zhengzhou, China.
  • Wang H; Zhongke Technology Achievement Transfer and Transformation Center of Henan Province, Zhengzhou, China.
  • Liu Y; Zhengzhou Institute of Biomedical Engineering and Technology, Zhengzhou, China.
  • Wang D; Zhongke Technology Achievement Transfer and Transformation Center of Henan Province, Zhengzhou, China.
  • Wu Z; Zhengzhou Institute of Biomedical Engineering and Technology, Zhengzhou, China.
  • Dong WF; Zhongke Technology Achievement Transfer and Transformation Center of Henan Province, Zhengzhou, China.
  • Zan M; CAS Key Laboratory of Biomedical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science (CAS), Suzhou, China.
Front Bioeng Biotechnol ; 11: 1308725, 2023.
Article en En | MEDLINE | ID: mdl-38169725
ABSTRACT

Introduction:

Pregnanediol-3-glucuronide (PdG), as the main metabolite of progesterone in urine, plays a significant role in the prediction of ovulation, threatened abortion, and menstrual cycle maintenance.

Methods:

To achieve a rapid and sensitive assay, we have designed a competitive model-based time-resolved fluorescence microsphere-lateral flow immunochromatography (TRFM-LFIA) strip.

Results:

The optimized TRFM-LFIA strip exhibited a wonderful response to PdG over the range of 30-2,000 ng/mL, the corresponding limit of detection (LOD) was calculated as low as 8.39 ng/mL. More importantly, the TRFM-LFIA strip was innovatively used for the quantitative detection of PdG in urine sample, and excellent recovery results were also obtained, ranging from 97.39% to 112.64%.

Discussion:

The TRFMLFIA strip possessed robust sensitivity and selectivity in the determination of PdG, indicating the great potential of being powerful tools in the biomedical and diagnosis region.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Front Bioeng Biotechnol Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Front Bioeng Biotechnol Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza