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Fluorescent in situ hybridization has limitations in screening NRG1 gene rearrangements.
Zhang, Xiaomei; Li, Lin; Gao, Fuping; Liu, Binbin; Li, Jing; Ren, Shuang; Peng, Shuangshuang; Qiu, Wei; Pu, Xiaohong; Ye, Qing.
Afiliación
  • Zhang X; Department of Pathology, Nanjing Jiangning Hospital, Nanjing, 211100, Jiangsu Province, China.
  • Li L; Department of Pathology, The Affiliated Drum Tower Hospital of Medical School,Nanjing University, Nanjing, 210008, Jiangsu Province, China.
  • Gao F; Department of Pathology, Nanjing Gaochun People's Hospital, Nanjing, 210008, Jiangsu Province, China.
  • Liu B; Department of Pathology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210008, Jiangsu Province, China.
  • Li J; Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210008, Jiangsu Province, China.
  • Ren S; Berry Oncology Corporation, Beijing, 100102, China.
  • Peng S; Department of Pathology, The Affiliated Drum Tower Hospital of Medical School,Nanjing University, Nanjing, 210008, Jiangsu Province, China.
  • Qiu W; Department of Pathology, The Affiliated Drum Tower Hospital of Medical School,Nanjing University, Nanjing, 210008, Jiangsu Province, China.
  • Pu X; Department of Pathology, Nanjing Jiangning Hospital, Nanjing, 211100, Jiangsu Province, China. 308012119@qq.com.
  • Ye Q; Department of Pathology, The Affiliated Drum Tower Hospital of Medical School,Nanjing University, Nanjing, 210008, Jiangsu Province, China. xiaohongpu06@163.com.
Diagn Pathol ; 19(1): 1, 2024 Jan 03.
Article en En | MEDLINE | ID: mdl-38173003
ABSTRACT

BACKGROUND:

NRG1 fusion is a promising therapeutic target for various tumors but its prevalence is extremely low, and there are no standardized testing algorithms for genetic assessment. MOTHODS In this study, we analyzed 3008 tumors using Fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) to screen for NRG1 translocation and p-HER3 expression.

RESULTS:

Our results demonstrated no cases with p-HER3 positivity through IHC. Nonetheless, 29 cases (0.96%) were identified positive for NRG1 translocation through FISH, with three different signal types. FISH-positive cases were subsequently subjected to next-generation sequencing (NGS) testing. However, only eight of these cases were confirmed with NRG1 fusion through NGS. Notably, we divided FISH into three types and FISH type C group was consistent with NGS results. All NGS NRG1 fusion tumors were adenocarcinomas, with a higher prevalence in females. Our findings indicate that although FISH has limitations in screening NRG1 gene rearrangements, NRG1 fusions can be reliably detected with signals exhibiting low copy numbers of the 5'-end of the gene and no fusion signals.

CONCLUSION:

Considering the high cost of NGS, FISH remains a useful method for screening NRG1 fusions in various types of tumors. This study provides valuable insights into the molecular mechanisms of NRG1 fusion and identifies potential treatment targets for patients suffering from this disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Adenocarcinoma / Neoplasias Pulmonares Tipo de estudio: Diagnostic_studies / Prognostic_studies / Risk_factors_studies / Screening_studies Límite: Female / Humans Idioma: En Revista: Diagn Pathol Asunto de la revista: PATOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Adenocarcinoma / Neoplasias Pulmonares Tipo de estudio: Diagnostic_studies / Prognostic_studies / Risk_factors_studies / Screening_studies Límite: Female / Humans Idioma: En Revista: Diagn Pathol Asunto de la revista: PATOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido