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Generation of two induced pluripotent stem cell lines from two sporadic amyotrophic lateral sclerosis patients.
Wen, Shan; Fu, Shujie; Gao, Chong; Lei, Kai; Liu, Xiaodong.
Afiliación
  • Wen S; School of Life Sciences, Westlake University, Hangzhou, China; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China; Westlake Institute for Advanced Study, Hangzhou, China.
  • Fu S; School of Life Sciences, Westlake University, Hangzhou, China; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China; Westlake Institute for Advanced Study, Hangzhou, China.
  • Gao C; School of Life Sciences, Westlake University, Hangzhou, China; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China; Westlake Institute for Advanced Study, Hangzhou, China.
  • Lei K; School of Life Sciences, Westlake University, Hangzhou, China; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China; Westlake Institute for Advanced Study, Hangzhou, China.
  • Liu X; School of Life Sciences, Westlake University, Hangzhou, China; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, China; Westlake Institute for Advanced Study, Hangzhou, China. Electronic address: liuxiaodong@westlake.edu.cn.
Stem Cell Res ; 74: 103288, 2024 02.
Article en En | MEDLINE | ID: mdl-38181637
ABSTRACT
Peripheral blood mononuclear cells were obtained from two patients diagnosed with amyotrophic lateral sclerosis (ALS), a 47-year-old female and a 45-year-old male. Induced pluripotent stem cells (iPSCs) were generated using a non-integrating SeV-based method, delivering the transcription factors OCT4, SOX2, c-MYC, and KLF4. These transgene-free iPSC lines exhibited typical pluripotent cell morphology, expressed pluripotency-associated markers, and had tri-lineage differentiation potential. Both iPSC lines were free of mycoplasma contamination and displayed normal karyotypes. The availability of these two cell lines provides a promising opportunity to use sporadic ALS models for investigating the intricate pathological mechanisms of ALS.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Pluripotentes Inducidas / Esclerosis Amiotrófica Lateral Tipo de estudio: Prognostic_studies Límite: Female / Humans / Male / Middle aged Idioma: En Revista: Stem Cell Res Año: 2024 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Pluripotentes Inducidas / Esclerosis Amiotrófica Lateral Tipo de estudio: Prognostic_studies Límite: Female / Humans / Male / Middle aged Idioma: En Revista: Stem Cell Res Año: 2024 Tipo del documento: Article País de afiliación: China