Protocol for assessing total antioxidant capacity in minimal volumes of varying clinical human samples.
STAR Protoc
; 5(1): 102822, 2024 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-38194341
ABSTRACT
Total antioxidant capacity (TAC), representative of the capacity to combat oxidative stress, is closely linked to numerous diseases. Here, we present a protocol for measuring TAC using minimal samples that are stable across varying pH levels and at room temperature. We describe steps for preparing and loading samples and working solutions and conducting and analyzing the colorimetric reaction. Sample sources include aqueous humor, vitreous, tears, and plasma, which allow the protocol to be used in various clinical diagnostic settings. For complete details on the use and execution of this protocol, please refer to publications by Tsao et al. (2022).1,2.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Colorimetría
/
Antioxidantes
Tipo de estudio:
Guideline
Límite:
Humans
Idioma:
En
Revista:
STAR Protoc
/
STAR protocols
Año:
2024
Tipo del documento:
Article
Pais de publicación:
Estados Unidos