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In vitro evaluation of enterococcus faecalis growth in different conditions on dentinal substrate.
Hage, Wajih; Karam Sarkis, Dolla; Kallassy, Mireille; Mallah, May; Zogheib, Carla.
Afiliación
  • Hage W; Departement of Endodontics, Saint Joseph University, Beirut, Lebanon.
  • Karam Sarkis D; Departement of Microbiology, Saint Joseph University, Beirut, Lebanon.
  • Kallassy M; Departement of Sciences, Saint Joseph University, Beirut, Lebanon.
  • Mallah M; Departement of Microbiology, Saint Joseph University, Beirut, Lebanon.
  • Zogheib C; Departement of Endodontics, Saint Joseph University, Beirut, Lebanon.
Biomater Investig Dent ; 10(1): 2287668, 2023.
Article en En | MEDLINE | ID: mdl-38204472
ABSTRACT
The aim of this study was to find the best growth conditions of Enterococcus faecalis on a dentinal substrate in order to be used for the development of a complex multispecies endodontic biofilm. Fifty two single rooted extracted human teeth and fifty two dentinal disks were mechanically prepared, sterilized, inoculated with Enterococcus faecalis and divided randomly into 8 groups where the substrate, the inoculation technique, the medium type, and the pre-treatment with collagen type I was varied. Bacterial count was evaluated and colonies were counted and confirmed by colony morphology observation on blood agar and Gram staining at 3,7, 14, 21, and 28 days. On day 14 of the culture, the bacterial count showed the highest values in all groups. Root canals and Type 1 collagen pre-treatment and glucose proved to have significant positive effects on the bacterial count compared to dentinal disks and BHI media only. The increase in bacterial count found with the direct inoculation technique was not significantly different from that of the indirect technique.

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomater Investig Dent Año: 2023 Tipo del documento: Article País de afiliación: Líbano Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomater Investig Dent Año: 2023 Tipo del documento: Article País de afiliación: Líbano Pais de publicación: Reino Unido