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Short chain fatty acids inhibit corneal inflammatory responses to TLR ligands via the ocular G-protein coupled receptor 43.
Wu, Jun; Chen, Nu; Grau, Elizabeth; Johnson, Luke; Liu, Yongqing; Li, Chi; Scott, Patrick A; Kim, Chang; Sun, Deming; Kaplan, Henry J; Shao, Hui.
Afiliación
  • Wu J; Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, School of Medicine, Louisville, KY, USA.
  • Chen N; Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, School of Medicine, Louisville, KY, USA.
  • Grau E; Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, School of Medicine, Louisville, KY, USA.
  • Johnson L; Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, School of Medicine, Louisville, KY, USA.
  • Liu Y; Department of Medicine-oncology, University of Louisville, School of Medicine, Louisville, KY, USA.
  • Li C; Department of Medicine-oncology, University of Louisville, School of Medicine, Louisville, KY, USA.
  • Scott PA; Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, School of Medicine, Louisville, KY, USA.
  • Kim C; Department of Pathology, Mary H. Weiser Food Allergy Center, 4025 Biomedical Science Research Building, 109 Zina Pitcher Place, Ann Arbor, MI, USA.
  • Sun D; Doheny Eye Institute & Department Ophthalmology, David Geffen School of Medicine/UCLA, Los Angeles, CA, USA.
  • Kaplan HJ; Department of Ophthalmology and Biochemistry & Molecular Biology, St. Louis University School of Medicine, St. Louis, Missouri, USA.
  • Shao H; Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, School of Medicine, Louisville, KY, USA. Electronic address: h0shao01@louisville.edu.
Ocul Surf ; 32: 48-57, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38224777
ABSTRACT

PURPOSE:

Short chain fatty acids (SCFAs) produced by gut microbiota are known to play primary roles in gut homeostasis by immunomodulation partially through G-protein coupled receptors (GPR) 43. Using mouse models of TLR ligand induced keratitis, we investigated whether SCFAs and GPR43 play any regulatory roles in the pathogenesis of inflammatory responses in the eye.

METHODS:

Both human and mouse eyes were labeled with a specific antibody for GPR43 and imaged by a laser scanning confocal microscope. Corneal cups from naïve C57BL/6J (B6) and GPR43 knockout (KO) mice were stimulated with TLR ligands in the presence or absence of sodium butyrate overnight and then processed for RT-PCR assay for expression of GPR43 and cytokines. Keratitis was induced by Poly IC in wild type (WT) B6, GPR43KO and chimeric mice and the disease severity was evaluated by the corneal fluorescein staining test, and infiltrating cell staining and calculating in corneal whole mount.

RESULTS:

GPR43 is expressed in both human and mouse eyes and the expression is bidirectionally regulated by TLR ligands and butyrate. Butyrate significantly inhibited inflammation caused by several TLR ligands such as Poly IC, Flagellin, and CpG-ODN (TLR-3, 5 and 9 agonists, respectively) in WT, but not GPR43KO, mice. Butyrate inhibition of TLR-induced keratitis is mediated by the GPR43 expressed in tissue but not hematopoietic, cells.

CONCLUSIONS:

This is the first report to demonstrate of the protective effect of SCFAs on microbial keratitis, and the dynamic expression and anti-inflammatory function of GPR43 in the eye. SCFAs can modulate inflammation and immunity in the eye through GPR43.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores Acoplados a Proteínas G / Modelos Animales de Enfermedad / Ácidos Grasos Volátiles / Queratitis Límite: Animals / Humans Idioma: En Revista: Ocul Surf Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores Acoplados a Proteínas G / Modelos Animales de Enfermedad / Ácidos Grasos Volátiles / Queratitis Límite: Animals / Humans Idioma: En Revista: Ocul Surf Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA