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'Green' silver nanoparticles combined with tyrosol as potential oral antimicrobial therapy.
Souza, José Antonio Santos; do Amaral, Jackeline Gallo; Monteiro, Douglas Roberto; Fernandes, Renan Aparecido; Fernandes, Gabriela Lopes; Gorup, Luiz Fernando; de Souza Neto, Francisco Nunes; de Camargo, Emerson Rodrigues; Agostinho, Alessandra Marçal; Barbosa, Debora Barros; Delbem, Alberto Carlos Botazzo.
Afiliación
  • Souza JAS; Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil.
  • do Amaral JG; Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil.
  • Monteiro DR; Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil.
  • Fernandes RA; Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil.
  • Fernandes GL; Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil.
  • Gorup LF; Department of Chemistry, Federal University of São Carlos, São Carlos 13565-905, São Paulo, Brazil.
  • de Souza Neto FN; Department of Chemistry, Federal University of São Carlos, São Carlos 13565-905, São Paulo, Brazil.
  • de Camargo ER; Department of Chemistry, Federal University of São Carlos, São Carlos 13565-905, São Paulo, Brazil.
  • Agostinho AM; Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48823, USA.
  • Barbosa DB; Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil.
  • Delbem ACB; Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil. Electronic address: alberto.delbem@unesp.br.
J Dent ; 143: 104867, 2024 04.
Article en En | MEDLINE | ID: mdl-38286192
ABSTRACT

OBJECTIVES:

This study aimed to evaluate silver nanoparticles (AgNPs) obtained by a 'green' route associated or not to tyrosol (TYR) against Streptococcus mutans and Candida albicans in planktonic and biofilms states.

METHODS:

AgNPs were obtained by a 'green' route using pomegranate extract. The minimum inhibitory concentration (MIC) against S. mutans and C. albicans was determined for AgNPs and TYR combined and alone, and fractional inhibitory concentration index (FICI) was calculated. Single biofilms of C. albicans and S. mutans were cultivated for 24 h and then treated with drugs alone or in combination for 24 h.

RESULTS:

AgNPs and TYR were effective against C. albicans and S. mutans considering planktonic cells alone and combined. The MIC values obtained for C. albicans was 312.5 µg/mL (AgNPs) and 50 mM (TYR) and for S. mutans was 78.1 µg/mL (AgNPs) and 90 mM (TYR). The combination of these antimicrobial agents was also effective against both microorganisms 2.44 µg/mL/0.08 mM (AgNPs/TYR) for C. albicans and 39.05 µg/mL /1.25 mM (AgNPs/TYR) for S. mutans. However, synergism was observed only for C. albicans (FICI 0.008). When biofilm was evaluated, a reduction of 4.62 log10 was observed for S. mutans biofilm cells treated with AgNPs (p < 0.05, Tukey test). However, the addition of TYR to AgNPs did not improve their action against biofilm cells (p > 0.05). AgNPs combined with TYR demonstrated a synergistic effect against C. albicans biofilms.

CONCLUSIONS:

These findings suggest the potential use of AgNPs with or without TYR against C. albicans and S. mutans, important oral pathogens. CLINICAL

SIGNIFICANCE:

AgNPs obtained by a 'green' route combined or not with TYR can be an alternative to develop several types of oral antimicrobial therapies and biomaterials.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alcohol Feniletílico / Nanopartículas del Metal / Antiinfecciosos Idioma: En Revista: J Dent Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alcohol Feniletílico / Nanopartículas del Metal / Antiinfecciosos Idioma: En Revista: J Dent Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido