[Study on gene therapy for DPOAE and ABR threshold changes in adult Otofî-/- mice].
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
; 38(1): 49-56, 2024 Jan.
Article
en Zh
| MEDLINE
| ID: mdl-38297849
ABSTRACT
Objective:
This study aims to analyze the threshold changes in distortion product otoacoustic emissionsï¼DPOAEï¼ and auditory brainstem responseï¼ABRï¼ in adult Otofî-/- mice before and after gene therapy, evaluating its effectiveness and exploring methods for assessing hearing recovery post-treatment.Methods:
At the age of 4 weeks, adult Otofî-/- mice received an inner ear injection of a therapeutic agent containing intein-mediated recombination of the OTOF gene, delivered via dual AAV vectors through the round window membraneï¼RWMï¼. Immunofluorescence staining assessed the proportion of inner ear hair cells with restored otoferlin expression and the number of synapses.Statistical analysis was performed to compare the DPOAE and ABR thresholds before and after the treatment.Results:
AAV-PHP. eB demonstrates high transduction efficiency in inner ear hair cells. The therapeutic regimen corrected hearing loss in adult Otofî-/- mice without impacting auditory function in wild-type mice. The changes in DPOAE and ABR thresholds after gene therapy are significantly correlated at 16 kHz. Post-treatment,a slight increase in DPOAE was observeds,followed by a recovery trend at 2 months post-treatment.Conclusion:
Gene therapy significantly restored hearing in adult Otofî-/- mice, though the surgical delivery may cause transient hearing damage. Precise and gentle surgical techniques are essential to maximize gene therapy's efficacy.Palabras clave
Texto completo:
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pérdida Auditiva
/
Oído Interno
Límite:
Animals
Idioma:
Zh
Revista:
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
Año:
2024
Tipo del documento:
Article
Pais de publicación:
China