Protocol for detecting RBM33-binding sites in HEK293T cells using PAR-CLIP-seq.
STAR Protoc
; 5(1): 102855, 2024 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-38300798
ABSTRACT
RNA-binding proteins (RBPs) regulate gene expression both co-transcriptionally and post-transcriptionally. Here, we provide a protocol for photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation followed by next-generation sequencing (PAR-CLIP-seq). PAR-CLIP-seq is a transcriptome-scale technique for identifying in vivo binding sites of RBPs at the single-nucleotide level. We detail procedures for the establishment of FLAG-RBM33 stable cell line, the sequencing library preparation, and the data analysis.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Unión al ARN
/
Secuenciación de Inmunoprecipitación de Cromatina
Límite:
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos