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Metabolomic Profiles and Pathways in Osteoarthritic Human Cartilage: A Comparative Analysis with Healthy Cartilage.
Welhaven, Hope D; Welfley, Avery H; Brahmachary, Priyanka; Bergstrom, Annika R; Houske, Eden; Glimm, Matthew; Bothner, Brian; Hahn, Alyssa K; June, Ronald K.
Afiliación
  • Welhaven HD; Department of Chemistry & Biochemistry, Montana State University, Bozeman MT.
  • Welfley AH; Department of Mechanical & Industrial Engineering, Montana State University, Bozeman MT.
  • Brahmachary P; Department of Mechanical & Industrial Engineering, Montana State University, Bozeman MT.
  • Bergstrom AR; Department of Chemical & Biological Engineering, Villanova University, Villanova, PA.
  • Houske E; Department of Biological and Environmental Sciences, Carroll College, Helena, MT.
  • Glimm M; Department of Biological and Environmental Sciences, Carroll College, Helena, MT.
  • Bothner B; Department of Chemistry & Biochemistry, Montana State University, Bozeman MT.
  • Hahn AK; Department of Biological and Environmental Sciences, Carroll College, Helena, MT.
  • June RK; Department of Mechanical & Industrial Engineering, Montana State University, Bozeman MT.
bioRxiv ; 2024 Jan 25.
Article en En | MEDLINE | ID: mdl-38328065
ABSTRACT

Objective:

Osteoarthritis (OA) is a chronic joint disease with heterogenous metabolic pathology. To gain insight into OA-related metabolism, healthy and end-stage osteoarthritic cartilage were compared metabolically to uncover disease-associated profiles, classify OA-specific metabolic endotypes, and identify targets for intervention for the diverse populations of individuals affected by OA.

Design:

Femoral head cartilage (n=35) from osteoarthritis patients were collected post-total joint arthroplasty. Healthy cartilage (n=11) was obtained from a tissue bank. Metabolites from all cartilage samples were extracted and analyzed using liquid chromatography-mass spectrometry metabolomic profiling. Additionally, cartilage extracts were pooled and underwent fragmentation analysis for biochemical identification of metabolites.

Results:

Specific metabolites and metabolic pathways, including lipid- and amino acid pathways, were differentially regulated between osteoarthritis-derived and healthy cartilage. The detected alterations of amino acids and lipids highlight key differences in bioenergetic resources, matrix homeostasis, and mitochondrial alterations in osteoarthritis-derived cartilage compared to healthy. Moreover, metabolomic profiles of osteoarthritic cartilage separated into four distinct endotypes highlighting the heterogenous nature of OA metabolism and diverse landscape within the joint between patients.

Conclusions:

The results of this study demonstrate that human cartilage has distinct metabolomic profiles between healthy and end-stage osteoarthritis patients. By taking a comprehensive approach to assess metabolic differences between healthy and osteoarthritic cartilage, and within osteoarthritic cartilage alone, several metabolic pathways with distinct regulation patterns were detected. Additional investigation may lead to the identification of metabolites that may serve as valuable indicators of disease status or potential therapeutic targets.
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: BioRxiv Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: BioRxiv Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos