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Differential Gene Expression following DHX36/G4R1 Knockout Is Associated with G-Quadruplex Content and Cancer.
Gumina, Joseph M; Richardson, Adam E; Shojiv, Mahmudul Hasan; Chambers, Antonio E; Sandwith, Siara N; Reisinger, Michael A; Karns, Taylor J; Osborne, Tyler L; Alashi, Hasna N; Anderson, Quinn T; Sharlow, Meredith E; Seiler, Dylan C; Rogers, Evan M; Bartosik, Anna R; Smaldino, Melissa A; Vaughn, James P; Wang, Yuh-Hwa; Smaldino, Philip J; Haney, Robert A.
Afiliación
  • Gumina JM; Department of Biology, Ball State University, Muncie, IN 47306, USA.
  • Richardson AE; Department of Biology, Ball State University, Muncie, IN 47306, USA.
  • Shojiv MH; Department of Biology, Ball State University, Muncie, IN 47306, USA.
  • Chambers AE; Department of Biology, Ball State University, Muncie, IN 47306, USA.
  • Sandwith SN; Department of Biology, Ball State University, Muncie, IN 47306, USA.
  • Reisinger MA; Department of Biology, Ball State University, Muncie, IN 47306, USA.
  • Karns TJ; Department of Biology, Ball State University, Muncie, IN 47306, USA.
  • Osborne TL; Department of Biology, Ball State University, Muncie, IN 47306, USA.
  • Alashi HN; Department of Biology, Ball State University, Muncie, IN 47306, USA.
  • Anderson QT; Department of Biology, Ball State University, Muncie, IN 47306, USA.
  • Sharlow ME; Department of Biology, Ball State University, Muncie, IN 47306, USA.
  • Seiler DC; Department of Biology, Ball State University, Muncie, IN 47306, USA.
  • Rogers EM; Department of Biology, Ball State University, Muncie, IN 47306, USA.
  • Bartosik AR; School of Medicine, University of Virginia, Charlottesville, VA 22908, USA.
  • Smaldino MA; Department of Biology, Ball State University, Muncie, IN 47306, USA.
  • Vaughn JP; NanoMedica LLC, Winston-Salem, NC 27106, USA.
  • Wang YH; School of Medicine, University of Virginia, Charlottesville, VA 22908, USA.
  • Smaldino PJ; Department of Biology, Ball State University, Muncie, IN 47306, USA.
  • Haney RA; Department of Biology, Ball State University, Muncie, IN 47306, USA.
Int J Mol Sci ; 25(3)2024 Feb 01.
Article en En | MEDLINE | ID: mdl-38339029
ABSTRACT
G-quadruplexes (G4s) are secondary DNA and RNA structures stabilized by positive cations in a central channel formed by stacked tetrads of Hoogsteen base-paired guanines. G4s form from G-rich sequences across the genome, whose biased distribution in regulatory regions points towards a gene-regulatory role. G4s can themselves be regulated by helicases, such as DHX36 (aliases G4R1 and RHAU), which possess the necessary activity to resolve these stable structures. G4s have been shown to both positively and negatively regulate gene expression when stabilized by ligands, or through the loss of helicase activity. Using DHX36 knockout Jurkat cell lines, we identified widespread, although often subtle, effects on gene expression that are associated with the presence or number of observed G-quadruplexes in promoters or gene regions. Genes that significantly change their expression, particularly those that show a significant increase in RNA abundance under DHX36 knockout, are associated with a range of cellular functions and processes, including numerous transcription factors and oncogenes, and are linked to several cancers. Our work highlights the direct and indirect role of DHX36 in the transcriptome of T-lymphocyte leukemia cells and the potential for DHX36 dysregulation in cancer.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Helicasas DEAD-box / G-Cuádruplex / Neoplasias Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Helicasas DEAD-box / G-Cuádruplex / Neoplasias Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos
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