From Nucleosomes to Compartments: Physicochemical Interactions Underlying Chromatin Organization.
Annu Rev Biophys
; 53(1): 221-245, 2024 Jul.
Article
en En
| MEDLINE
| ID: mdl-38346246
ABSTRACT
Chromatin organization plays a critical role in cellular function by regulating access to genetic information. However, understanding chromatin folding is challenging due to its complex, multiscale nature. Significant progress has been made in studying in vitro systems, uncovering the structure of individual nucleosomes and their arrays, and elucidating the role of physicochemical forces in stabilizing these structures. Additionally, remarkable advancements have been achieved in characterizing chromatin organization in vivo, particularly at the whole-chromosome level, revealing important features such as chromatin loops, topologically associating domains, and nuclear compartments. However, bridging the gap between in vitro and in vivo studies remains challenging. The resemblance between in vitro and in vivo chromatin conformations and the relevance of internucleosomal interactions for chromatin folding in vivo are subjects of debate. This article reviews experimental and computational studies conducted at various length scales, highlighting the significance of intrinsic interactions between nucleosomes and their roles in chromatin folding in vivo.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cromatina
/
Nucleosomas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Annu Rev Biophys
Asunto de la revista:
BIOFISICA
Año:
2024
Tipo del documento:
Article
Pais de publicación:
Estados Unidos