Using an ImageJ-based script to detect replication stress and associated cell cycle exit from G2 phase by fluorescence microscopy.
Methods Cell Biol
; 182: 187-197, 2024.
Article
en En
| MEDLINE
| ID: mdl-38359976
ABSTRACT
Replication stress risks genomic integrity. Depending on the level, replication stress can lead to slower progression through S phase and entry into G2 phase with DNA damage. In G2 phase, cells either recover and eventually enter mitosis or permanently withdraw from the cell cycle. Here we describe a method to detect cell cycle distribution, replication stress and cell cycle exit from G2 phase using fluorescence microscopy. We provide a script to automate the analysis using ImageJ. The focus has been to make a script and setup that is accessible to people without extensive computer knowledge.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fase G2
/
Mitosis
Tipo de estudio:
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Methods Cell Biol
Año:
2024
Tipo del documento:
Article
Pais de publicación:
Estados Unidos