Determination of [Glu1]-fibrinopeptide B purity by gas chromatography - isotope dilution mass spectrometry.
Anal Methods
; 16(12): 1741-1747, 2024 Mar 22.
Article
en En
| MEDLINE
| ID: mdl-38372017
ABSTRACT
The present work assessed the purity of [Glu1]-fibrinopeptide B (GFB) as a model peptide using gas chromatography - isotope dilution mass spectrometry. GFB and various isotope-labeled amino acids were hydrolyzed in HCl and then derivatized using optimized procedures. The primary impurity in GFB was also identified and used to correct the final result. A method repeatability of 0.5% was achieved and linear calibrations were obtained for five amino acids. The LOD and LOQ were 0.041 to 0.096 µg g-1, and 0.16 to 0.56 µg g-1, respectively. The purity of GFB was found to be (0.715 ± 0.012) g g-1. This technique exhibited comparable accuracy to that obtainable from liquid chromatography - isotope dilution mass spectrometry but at lower cost. This method could be employed as a reference technique or in fields such as clinical diagnostics or bio-pharmaceutical peptide purity analysis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Péptidos
/
Fibrinopéptido B
Idioma:
En
Revista:
Anal Methods
Año:
2024
Tipo del documento:
Article
País de afiliación:
China