Rapid screening and target-guided isolation of antioxidants from German chamomile by 2,2-diphenyl-1-picrylhydrazyl-ultra-high-performance liquid chromatography-mass spectrometry coupled with off-line two-dimensional high-speed countercurrent chromatography.
J Sep Sci
; 47(4): e2300770, 2024 Feb.
Article
en En
| MEDLINE
| ID: mdl-38403448
ABSTRACT
German chamomile is one of the most effective herbal elements used in anti-allergic products and as an antioxidant. Herein, the antioxidant activity of different extract fractions of German chamomile was initially evaluated using an off-line 2,2-diphenyl-1-picrylhydrazyl spectrophotometric assay. The ethyl acetate extract demonstrated the highest efficacy in scavenging free radicals. Based on this, a rapid screening and separation method using ultra-high-performance liquid chromatography combined with the 2,2-diphenyl-1-picrylhydrazyl assay was implemented to identify antioxidants in the ethyl acetate fraction of German chamomile flowers. Ten potential radical scavengers were tentatively screened from German chamomile using a target-guided isolating approach with off-line two-dimensional high-speed countercurrent chromatography and the structures of the compounds were analyzed and identified. Ultimately, 10 radical scavengers were obtained from the ethyl acetate extract with a purity quotient exceeding 90%. The results demonstrated the effectiveness and reproducibility of this method for isolating potential antioxidants from complex mixtures in a targeted manner. This strategy can be applied to the target-guided isolation of complex mixtures of natural products with broad K-values and similar structures.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Picratos
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Compuestos de Bifenilo
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Distribución en Contracorriente
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Matricaria
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Acetatos
Idioma:
En
Revista:
J Sep Sci
Año:
2024
Tipo del documento:
Article
Pais de publicación:
Alemania