Ameliorative effect of salidroside on the cyclophosphamide-induced premature ovarian failure in a rat model.
Free Radic Res
; 58(2): 107-116, 2024 Feb.
Article
en En
| MEDLINE
| ID: mdl-38408280
ABSTRACT
BACKGROUND:
Oxidative stress injury is an important pathological factor of premature ovarian failure (POF). Salidroside, extracted from the Chinese herb-Rhodiola rosea, has advantages in antioxidant characteristics. However, their therapeutic efficacy and mechanisms in POF have not been explored.PURPOSE:
This study aims to assess the therapeutic effects of salidroside in chemotherapy-induced ovarian failure rats.METHODS:
A POF rat model was established by injection of cyclophosphamide, followed by treatment with salidroside. The therapeutic effect of salidroside was evaluated based on hormone levels, follicle count, and reproductive ability. Oxidative stress injury was assessed by the detection of SOD enzyme activity and MDA levels. Differential gene expression of Keap1, Nrf2, HMOX1, NQO1, AMH, BMP15, and GDF9, were identified by qRTPCR. The protein expression of Keap1, Nrf2, P53, and Bcl-2 were detected by western blot.RESULTS:
Salidroside treatment markedly restored FSH, E2, and AMH hormone secretion levels, reduced follicular atresia, and increased antral follicle numbers in POF rats. In addition, salidroside improves fertility in POF rats, activates the Nrf2 signaling pathway, and reduces the level of oxidative stress. The recovery function of high dose salidroside (50 mg/kg) in a reproductive assay was significantly improved than that of lower dose salidroside (25 mg/kg). Meanwhile, the safety evaluation of salidroside treatment in rats showed that salidroside was safe for POF rats at doses of 25-50 mg/kg.CONCLUSIONS:
Salidroside therapy improved premature ovarian failure significantly through antioxidant function and activating Nrf2 signaling.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fenoles
/
Insuficiencia Ovárica Primaria
/
Glucósidos
Límite:
Animals
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Female
/
Humans
Idioma:
En
Revista:
Free Radic Res
/
Free radic. res
/
Free radical research
Asunto de la revista:
BIOQUIMICA
Año:
2024
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Reino Unido