In utero pulse injection of isotopic amino acids quantifies protein turnover rates during murine fetal development.
Cell Rep Methods
; 4(2): 100713, 2024 Feb 26.
Article
en En
| MEDLINE
| ID: mdl-38412836
ABSTRACT
Protein translational control is critical for ensuring that the fetus develops correctly and that necessary organs and tissues are formed and functional. We developed an in utero method to quantify tissue-specific protein dynamics by monitoring amino acid incorporation into the proteome after pulse injection. Fetuses of pregnant mice were injected with isotopically labeled lysine and arginine via the vitelline vein at various embyonic days, and organs and tissues were harvested. By analyzing the nascent proteome, unique signatures of each tissue were identified by hierarchical clustering. In addition, the quantified proteome-wide turnover rates were calculated between 3.81E-5 and 0.424 h-1. We observed similar protein turnover profiles for analyzed organs (e.g., liver vs. brain); however, their distributions of turnover rates vary significantly. The translational kinetic profiles of developing organs displayed differentially expressed protein pathways and synthesis rates, which correlated with known physiological changes during mouse development.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteoma
/
Aminoácidos
Límite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Cell Rep Methods
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos