The Expression of miR-377-3p in Patients with DKD and the Regulatory Mechanism of miR-377-3p on the Inflammatory Response of HK-2 Cells Through TGF-ß.
Diabetes Metab Syndr Obes
; 17: 903-911, 2024.
Article
en En
| MEDLINE
| ID: mdl-38414866
ABSTRACT
Objective:
The purpose of the study was to investigate the expression levels and correlation of inflammatory factors such as miR-377-3p and TGF-ß in patients with diabetic kidney disease (DKD), and to investigate the regulatory mechanism of transfection of miR-377-3p on the inflammatory response of HK-2 cell induced by high glucose.Methods:
According to UACR, patients were divided into normal albuminuria group (Con, n = 29), microalbuminuria group (Micro, n = 31) and macroalbuminuria group (Macro, n = 30), analyzed the correlation and influencing factors between DKD and inflammatory factor. HK-2 cells were randomly divided into four groups normal control group (NC), high glucose group (HG), miR-377-3p overexpression group (MIN), and miR-377-3p inhibition group (IN). After transfection of miR-377-3p mimics and inhibitors, the contents of TGF-ß, IL-6 and IL-18 were detected by RT-PCR and Western blot.Results:
The levels of miR-377-3p, TGF-ß, IL-6 and IL-18 in both Micro group and Macro group were significantly higher than those in Con group (P < 0.05); Pearson correlation analysis showed that miR-377-3p was positively correlated with UACR, TG, TGF-ß, IL-6 and IL-18, and negatively correlated with GFR (P < 0.05). Cell experiment RT-PCR and Western blot results showed that miR-377-3p, TGF-ß, IL-6 and IL-18 in HG group were significantly higher than those in NC group (P < 0.05). After transfection with miR-377-3p inhibitor, the levels of miR-377-3p, TGF-ß, IL-6 and IL-18 in IN group were significantly decreased compared with HG group and MIN group.Conclusion:
miR-377-3p expression was elevated both in serum of DKD patients and in HK-2 cells with high glucose induced injury, overexpression of miR-377-3p exacerbates the damage to HK-2 cells and promotes the progression of DKD. Silencing miR-377-3p can potentially regulate the levels of inflammatory factors in HK-2 cells by targeting downregulation of TGF-ß expression, thereby mitigating the damage to HK-2 cells and delaying the development of diabetic kidney disease.
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Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Diabetes Metab Syndr Obes
Año:
2024
Tipo del documento:
Article