PSF-Radon transform algorithm: Measurement of the point-spread function from the Radon transform of the line-spread function.
Microsc Res Tech
; 87(7): 1507-1520, 2024 Jul.
Article
en En
| MEDLINE
| ID: mdl-38419356
ABSTRACT
In this article, we present a new method called point spread function (PSF)-Radon transform algorithm. This algorithm consists on recovering the instrument PSF from the Radon transform (in the line direction axis) of the line spread function (i.e., the image of a line). We present the method and tested with synthetic images, and real images from macro lens camera and microscopy. A stand-alone program along with a tutorial is available, for any interested user, in Martinez (PSF-Radon transform algorithm, standalone program). RESEARCH HIGHLIGHTS Determining the instrument PSF is a key issue. Precise PSF determinations are mandatory if image improvement is performed numerically by deconvolution. Much less exposure time to achieve the same performance than a measurement of the PSF from a very small bead. Does not require having to adjust the PSF by an analytical function to overcome the noise uncertainties.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Microsc Res Tech
Asunto de la revista:
DIAGNOSTICO POR IMAGEM
Año:
2024
Tipo del documento:
Article
País de afiliación:
Argentina