Your browser doesn't support javascript.
loading
Investigations on Microbes Attached to the Cuticle of Phytonematodes.
Topalovic, Olivera; Radakovic, Zoran S; Elhady, Ahmed; Bredenbruch, Sandra; Heuer, Holger.
Afiliación
  • Topalovic O; Department of Biology, Section of Terrestrial Ecology, University of Copenhagen, Copenhagen, Denmark. olivera.topalovic@bio.ku.dk.
  • Radakovic ZS; Laboratory of Insect and Nematode Management, Corteva Agriscience Research, Center Eschbach, Eschbach, Germany.
  • Elhady A; Epidemiology and Pathogen Diagnostics, Julius Kuehn Institute, Braunschweig, Germany.
  • Bredenbruch S; Rheinische Friedrich-Wilhelms-University of Bonn, INRES-Molecular Phytomedicine, Bonn, Germany.
  • Heuer H; Epidemiology and Pathogen Diagnostics, Julius Kuehn Institute, Braunschweig, Germany.
Methods Mol Biol ; 2756: 257-270, 2024.
Article en En | MEDLINE | ID: mdl-38427298
ABSTRACT
Nematodes form various associations with soil microbiome. Experimental studies on nematode-attached microbes can improve mechanistic understanding of these associations and lead to new discoveries relevant for the field of nematode biocontrol. Microbial attachment to the surface of phytonematodes is very specific and influenced by a multitude of factors, including the designation of nematodes and microbes, environmental and biological factors in soil, time of incubation, and the ratio and evolutionary trajectories between nematodes and microbes. Here, we describe how the classical nematological and microbiological techniques can be coupled with the advanced molecular tools to study the microbial attachment to phytonematodes in soil. We focus on the characterization of nematode-attached microbes using classical microbiological approaches and high-throughput amplicon sequencing and on the effects of nematode-attached microbes on plant defense responses.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbiota / Nematodos Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Dinamarca

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microbiota / Nematodos Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Dinamarca