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Allergenicity and structural properties of new Cor a 1 isoallergens from hazel identified in different plant tissues.
Hendrich, Julian M; Reuter, Andreas; Jacob, Thessa P; Kara, Hatice; Amer, Sherine; Rödel, Katharina; Wöhrl, Birgitta M.
Afiliación
  • Hendrich JM; Lehrstuhl Biochemie IV - Biophysikalische Chemie, Universität Bayreuth, Universitätsstrasse 30, 95447, Bayreuth, Germany.
  • Reuter A; Division of Allergology, Paul-Ehrlich-Institut, 63225, Langen, Germany.
  • Jacob TP; Lehrstuhl Biochemie IV - Biophysikalische Chemie, Universität Bayreuth, Universitätsstrasse 30, 95447, Bayreuth, Germany.
  • Kara H; Lehrstuhl Biochemie IV - Biophysikalische Chemie, Universität Bayreuth, Universitätsstrasse 30, 95447, Bayreuth, Germany.
  • Amer S; Lehrstuhl Biochemie IV - Biophysikalische Chemie, Universität Bayreuth, Universitätsstrasse 30, 95447, Bayreuth, Germany.
  • Rödel K; Lehrstuhl Biochemie IV - Biophysikalische Chemie, Universität Bayreuth, Universitätsstrasse 30, 95447, Bayreuth, Germany.
  • Wöhrl BM; Lehrstuhl Biochemie IV - Biophysikalische Chemie, Universität Bayreuth, Universitätsstrasse 30, 95447, Bayreuth, Germany. birgitta.woehrl@uni-bayreuth.de.
Sci Rep ; 14(1): 5618, 2024 03 07.
Article en En | MEDLINE | ID: mdl-38454094
ABSTRACT
The hazel allergen Cor a 1 is a PR-10 protein, closely related to the major birch pollen allergen Bet v 1. Hazel allergies are caused by cross-reactive IgE antibodies originally directed against Bet v 1. Despite the importance of PR-10 proteins in allergy development, their function and localization in the plant remain largely elusive. Therefore, the presence of Cor a 1 mRNA and proteins was investigated in different tissues, i.e., the female flower, immature and mature nuts, catkins, and pollen. Four yet unknown Cor a 1 isoallergens, i.e., Cor a 1.0501-1.0801, and one new Cor a 1.03 variant were discovered and characterized. Depending on the isoallergen, the occurrence and level of mRNA expression varied in different tissues, suggesting different functions. Interestingly, Cor a 1.04 previously thought to be only present in nuts, was also detected in catkins and pollen. The corresponding Cor a 1 genes were expressed in Escherichia coli. The purified proteins were analysed by CD and NMR spectroscopy. Immunoblots and ELISAs to determine their allergenic potential showed that the new proteins reacted positively with sera from patients allergic to birch, hazel and elder pollen and were recognized as novel isoallergens/variants by the WHO/IUIS Allergen Nomenclature Sub-Committee.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Corylus / Hipersensibilidad Límite: Aged / Humans Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Corylus / Hipersensibilidad Límite: Aged / Humans Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido