Your browser doesn't support javascript.
loading
Data-Independent Acquisition and Label-Free Quantification for Quantitative Proteomics Analysis of Human Cerebrospinal Fluid.
Makepeace, Karl A T; Rookyard, Alexander W; Das, Lipi; Vardarajan, Badri N; Chakrabarty, Jayanta K; Jain, Anu; Kang, Min Suk; Werth, Emily G; Reyes-Dumeyer, Dolly; Zerlin-Esteves, Marielba; Honig, Lawrence S; Mayeux, Richard; Brown, Lewis M.
Afiliación
  • Makepeace KAT; Department of Biological Sciences, Quantitative Proteomics and Metabolomics Center, Columbia University, New York, New York.
  • Rookyard AW; Department of Biological Sciences, Quantitative Proteomics and Metabolomics Center, Columbia University, New York, New York.
  • Das L; Department of Biological Sciences, Quantitative Proteomics and Metabolomics Center, Columbia University, New York, New York.
  • Vardarajan BN; Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Vagelos College of Physicians and Surgeons, Columbia University, New York, New York.
  • Chakrabarty JK; Department of Biological Sciences, Quantitative Proteomics and Metabolomics Center, Columbia University, New York, New York.
  • Jain A; Department of Biological Sciences, Quantitative Proteomics and Metabolomics Center, Columbia University, New York, New York.
  • Kang MS; Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Vagelos College of Physicians and Surgeons, Columbia University, New York, New York.
  • Werth EG; Department of Biological Sciences, Quantitative Proteomics and Metabolomics Center, Columbia University, New York, New York.
  • Reyes-Dumeyer D; Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Vagelos College of Physicians and Surgeons, Columbia University, New York, New York.
  • Zerlin-Esteves M; The Gertrude H. Sergievsky Center, Vagelos College of Physicians and Surgeons, Columbia University, New York, New York.
  • Honig LS; The Gertrude H. Sergievsky Center, Vagelos College of Physicians and Surgeons, Columbia University, New York, New York.
  • Mayeux R; Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Vagelos College of Physicians and Surgeons, Columbia University, New York, New York.
  • Brown LM; The Gertrude H. Sergievsky Center, Vagelos College of Physicians and Surgeons, Columbia University, New York, New York.
Curr Protoc ; 4(3): e1014, 2024 Mar.
Article en En | MEDLINE | ID: mdl-38506436
ABSTRACT
This article presents a practical guide to mass spectrometry-based data-independent acquisition and label-free quantification for proteomics analysis applied to cerebrospinal fluid, offering a robust and scalable approach to probing the proteomic composition of the central nervous system. © 2024 Wiley Periodicals LLC. Basic Protocol 1 Cerebrospinal fluid sample collection and preparation for mass spectrometry analysis Basic Protocol 2 Mass spectrometry sample analysis with data-independent acquisition Support Protocol Data-dependent mass spectrometry and spectral library construction Basic Protocol 3 Analysis of mass spectrometry data.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteoma / Proteómica Límite: Humans Idioma: En Revista: Curr Protoc Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteoma / Proteómica Límite: Humans Idioma: En Revista: Curr Protoc Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos