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In vitro cultivation of Vismia japurensis: Isolation of the new anthrone 1,8,10-trihydroxy-3,10-dimethyl-9(10H)-anthracenone.
Lima, Laísley M; Silva, Weison L; Souza, Julio Cezar DE; Nunez, Cecilia V.
Afiliación
  • Lima LM; Laboratório de Bioprospecção e Biotecnologia, Coordenação de Tecnologia e Inovação, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Petrópolis, 69037-375 Manaus, AM, Brazil.
  • Silva WL; Laboratório de Bioprospecção e Biotecnologia, Coordenação de Tecnologia e Inovação, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Petrópolis, 69037-375 Manaus, AM, Brazil.
  • Souza JC; Laboratório de Bioprospecção e Biotecnologia, Coordenação de Tecnologia e Inovação, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Petrópolis, 69037-375 Manaus, AM, Brazil.
  • Nunez CV; Laboratório de Bioprospecção e Biotecnologia, Coordenação de Tecnologia e Inovação, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Petrópolis, 69037-375 Manaus, AM, Brazil.
An Acad Bras Cienc ; 96(1): e20230456, 2024.
Article en En | MEDLINE | ID: mdl-38511745
ABSTRACT
Vismia japurensis Reichardt is a plant of ecological and chemical importance from which a variety of bioactive substances have been isolated. The current study aimed to establish in vitro cultures of this species as a source of secondary metabolites. Appropriate decontamination treatments and germination tests were performed and, after in vitro culture establishment, the propagated plants were multiplied in a sterile environment to increase the biomass of available experimental material. Seeds showed low contamination and a high germination percentage on Woody Plant Medium (WPM) supplemented with gibberellic acid (both at concentrations of 5 and 10 mg/L). V. japurensis nodal segments rapidly regenerated when first grown in WPM and then transplanted to Murashige and Skoog medium (MS). After 60 days in MS medium, the propagated plants were removed, lyophilized, and extracted with hexane and methanol. The hexane extract was fractionated via open column chromatography, and the substance isolated was purified by high performance liquid chromatography. Structural determination of the isolated substance was carried out using one and two-dimensional nuclear magnetic resonance and mass spectrometry. The isolated substance was identified as 1,8,10-trihydroxy-3,10-dimethyl-9(10H)-anthracenone, which, based on the conducted literature search, is reported for the first time.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plantas / Antronas / Hexanos Idioma: En Revista: An Acad Bras Cienc Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plantas / Antronas / Hexanos Idioma: En Revista: An Acad Bras Cienc Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil