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Protocol for generating postganglionic sympathetic neurons using human pluripotent stem cells for electrophysiological and functional assessments.
Wu, Hsueh-Fu; Art, Jennifer; Saini, Tripti; Zeltner, Nadja.
Afiliación
  • Wu HF; Center for Molecular Medicine, University of Georgia, Athens, GA 30602, USA; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA.
  • Art J; Center for Molecular Medicine, University of Georgia, Athens, GA 30602, USA; Biomedical and Translational Sciences Institute, Neuroscience Program, University of Georgia, Athens, GA 30602, USA.
  • Saini T; Center for Molecular Medicine, University of Georgia, Athens, GA 30602, USA; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA.
  • Zeltner N; Center for Molecular Medicine, University of Georgia, Athens, GA 30602, USA; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA; Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA. Electronic address: nadja.zeltner@uga.edu.
STAR Protoc ; 5(2): 102970, 2024 Jun 21.
Article en En | MEDLINE | ID: mdl-38517897
ABSTRACT
Assessing the development and function of the sympathetic nervous system in diseases on a large scale is challenging. Here, we present a protocol to generate human pluripotent stem cell (hPSC)-derived postganglionic sympathetic neurons (symNs) differentiated via neural crest cells (NCCs), which can be cryopreserved. We describe steps for hPSC replating, NCC replating and cryobanking, and symN differentiation. We then demonstrate the functionality of the hPSC-derived symNs, focusing on electrophysiological activity, calcium flux, and norepinephrine dynamics. For complete details on the use and execution of this protocol, please refer to Wu et al.1,2.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Células Madre Pluripotentes / Neuronas Límite: Humans Idioma: En Revista: STAR Protoc / STAR protocols Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Células Madre Pluripotentes / Neuronas Límite: Humans Idioma: En Revista: STAR Protoc / STAR protocols Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos