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Iron regulatory protein from the hard tick Haemaphysalis longicornis: characterization, function and assessment as a protective antigen.
Wang, Duo; Zhang, Xiaojing; Li, Hongxia; Wang, Ting; Ma, Xiaojin; Yu, Zhijun; Wang, Fang; Zhang, Yankai; Liu, Jingze.
Afiliación
  • Wang D; Ministry of Education Key Laboratory of Molecular and Cellular Biology; Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology; Hebei Collaborative Innovation Center for Eco-Environment; Hebei Research Center of the Basic Discipline of Cellular Biology, College of Life Science
  • Zhang X; Ministry of Education Key Laboratory of Molecular and Cellular Biology; Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology; Hebei Collaborative Innovation Center for Eco-Environment; Hebei Research Center of the Basic Discipline of Cellular Biology, College of Life Science
  • Li H; Ministry of Education Key Laboratory of Molecular and Cellular Biology; Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology; Hebei Collaborative Innovation Center for Eco-Environment; Hebei Research Center of the Basic Discipline of Cellular Biology, College of Life Science
  • Wang T; Ministry of Education Key Laboratory of Molecular and Cellular Biology; Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology; Hebei Collaborative Innovation Center for Eco-Environment; Hebei Research Center of the Basic Discipline of Cellular Biology, College of Life Science
  • Ma X; Ministry of Education Key Laboratory of Molecular and Cellular Biology; Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology; Hebei Collaborative Innovation Center for Eco-Environment; Hebei Research Center of the Basic Discipline of Cellular Biology, College of Life Science
  • Yu Z; Ministry of Education Key Laboratory of Molecular and Cellular Biology; Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology; Hebei Collaborative Innovation Center for Eco-Environment; Hebei Research Center of the Basic Discipline of Cellular Biology, College of Life Science
  • Wang F; Ministry of Education Key Laboratory of Molecular and Cellular Biology; Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology; Hebei Collaborative Innovation Center for Eco-Environment; Hebei Research Center of the Basic Discipline of Cellular Biology, College of Life Science
  • Zhang Y; Ministry of Education Key Laboratory of Molecular and Cellular Biology; Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology; Hebei Collaborative Innovation Center for Eco-Environment; Hebei Research Center of the Basic Discipline of Cellular Biology, College of Life Science
  • Liu J; Ministry of Education Key Laboratory of Molecular and Cellular Biology; Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology; Hebei Collaborative Innovation Center for Eco-Environment; Hebei Research Center of the Basic Discipline of Cellular Biology, College of Life Science
Pest Manag Sci ; 80(8): 3922-3934, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38520319
ABSTRACT

BACKGROUND:

Ticks are blood-feeding ectoparasites with different host specificities and are capable of pathogen transmission. Iron regulatory proteins (IRPs) play crucial roles in iron homeostasis in vertebrates. However, their functions in ticks remain poorly understood. The aim of the present study was to investigate the characteristics, functions, molecular mechanisms, and the vaccine efficacy of IRP in the hard tick Haemaphysalis longicornis.

RESULTS:

The full-length complementary DNA of IRP from Haemaphysalis longicornis (HlIRP) was 2973 bp, including a 2772 bp open reading frame. It is expressed throughout three developmental stages (larvae, nymphs, and adult females) and in various tissues (salivary glands, ovaries, midgut, and Malpighian tubules). Recombinant Haemaphysalis longicornis IRP (rHlIRP) was obtained via a prokaryotic expression system and exhibited aconitase, iron chelation, radical-scavenging, and hemolytic activities in vitro. RNA interference-mediated IRP knockdown reduced tick engorgement weight, ovary weight, egg mass weight, egg hatching rate, and ovary vitellin content, as well as prolonging the egg incubation period. Proteomics revealed that IRP may affect tick reproduction and development through proteasome pathway-associated, ribosomal, reproduction-related, and iron metabolism-related proteins. A trial on rabbits against adult Haemaphysalis longicornis infestation demonstrated that rHlIRP vaccine could significantly decrease engorged weight (by 10%), egg mass weight (by 16%) and eggs hatching rate (by 22%) of ticks. The overall immunization efficacy using rHlIRP against adult females was 41%.

CONCLUSION:

IRP could limit reproduction and development in Haemaphysalis longicornis, and HlIRP was confirmed as a candidate vaccine antigen to impair tick iron metabolism and protect the host against tick infestation. © 2024 Society of Chemical Industry.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Reguladoras del Hierro / Proteínas de Artrópodos / Haemaphysalis longicornis Límite: Animals Idioma: En Revista: Pest Manag Sci Asunto de la revista: TOXICOLOGIA Año: 2024 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Reguladoras del Hierro / Proteínas de Artrópodos / Haemaphysalis longicornis Límite: Animals Idioma: En Revista: Pest Manag Sci Asunto de la revista: TOXICOLOGIA Año: 2024 Tipo del documento: Article