Comparison and optimization of protocols and whole-genome capture conditions for ancient DNA samples.
Biotechniques
; 76(5): 216-223, 2024 May.
Article
en En
| MEDLINE
| ID: mdl-38530148
ABSTRACT
Ancient DNA (aDNA) obtained from human remains is typically fragmented and present in relatively low amounts. Here we investigate a set of optimal methods for producing aDNA data by comparing silica-based DNA extraction and aDNA library preparation protocols. We also test the efficiency of whole-genome enrichment (WGC) on ancient human samples by modifying a number of parameter combinations. We find that the Dabney extraction protocol performs significantly better than alternatives. We further observed a positive trend with the BEST library protocol indicating lower clonality. Notably, our results suggest that WGC is effective at retrieving endogenous DNA, particularly from poorly-preserved human samples, by increasing human endogenous proportions by 5x. Thus, aDNA studies will be most likely to benefit from our results.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Genoma Humano
/
ADN Antiguo
Límite:
Humans
Idioma:
En
Revista:
Biotechniques
Año:
2024
Tipo del documento:
Article
País de afiliación:
Suecia