Efflux Pump Inhibitor Potentiates the Antimicrobial Photodynamic Inactivation of Multidrug-Resistant Acinetobacter baumannii.
Photobiomodul Photomed Laser Surg
; 42(4): 314-320, 2024 Apr.
Article
en En
| MEDLINE
| ID: mdl-38536111
ABSTRACT
Background:
Acinetobacter baumannii, a nosocomial pathogen, poses a major public health problem due to generating resistance to several antimicrobial agents. Antimicrobial photodynamic inactivation (APDI) employs a nontoxic dye as a photosensitizer (PS) and light to produce reactive oxygen species that destroy bacterial cells. The intracellular concentration of PS could be affected by factors such as the function of efflux pumps to emit PS from the cytosol.Objective:
To evaluate the augmentation effect of an efflux pump inhibitor, verapamil, three multidrug-resistant A. baumannii were subjected to APDI by erythrosine B (EB). Methods andresults:
The combination of EB and verapamil along with irradiation at 530 nm induced a lethal effect and more than 3 log colony-forming unit reduction to all A. baumannii strains in planktonic state. In contrast, EB and irradiation alone could produce only a sublethal effect on two of the strains.Conclusions:
These data suggest that verapamil increases the intracellular concentration of EB, which potentiates the lethal efficacy of APDI. Verapamil could be applied with EB and green light to improve their antimicrobial efficacy against A. baumannii-localized infections.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Verapamilo
/
Fármacos Fotosensibilizantes
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Farmacorresistencia Bacteriana Múltiple
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Acinetobacter baumannii
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Eritrosina
/
Colorantes Fluorescentes
Idioma:
En
Revista:
Photobiomodul Photomed Laser Surg
Año:
2024
Tipo del documento:
Article
País de afiliación:
Irán