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Indole-3-carbinol ameliorates ovarian damage in female old mice through Nrf2/HO-1 pathway activation.
Hu, Huiqing; Li, Fangfang; Zhu, Fengyu; Li, Jun; Wang, Siyuan; He, Zhuoying; Chen, Jiaqi; Cheng, Linghui; Zhong, Fei.
Afiliación
  • Hu H; Department of Oncology, Fuyang Hospital of Anhui Medical University, Fuyang, 236000, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University), No 81 Meishan Road, Hefei 230032, Anhui, China.
  • Li F; Department of Oncology, Fuyang Hospital of Anhui Medical University, Fuyang, 236000, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University), No 81 Meishan Road, Hefei 230032, Anhui, China.
  • Zhu F; Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No 218 Jixi Road, Hefei 230022, Anhui, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University), No 81 Meishan Road, Hefei 230032, Anhui, China; Key Lab
  • Li J; Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No 218 Jixi Road, Hefei 230022, Anhui, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University), No 81 Meishan Road, Hefei 230032, Anhui, China; Key Lab
  • Wang S; Department of Oncology, Fuyang Hospital of Anhui Medical University, Fuyang, 236000, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University), No 81 Meishan Road, Hefei 230032, Anhui, China.
  • He Z; Department of Oncology, Fuyang Hospital of Anhui Medical University, Fuyang, 236000, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University), No 81 Meishan Road, Hefei 230032, Anhui, China.
  • Chen J; Department of Oncology, Fuyang Hospital of Anhui Medical University, Fuyang, 236000, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University), No 81 Meishan Road, Hefei 230032, Anhui, China.
  • Cheng L; Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No 218 Jixi Road, Hefei 230022, Anhui, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University), No 81 Meishan Road, Hefei 230032, Anhui, China; Key Lab
  • Zhong F; Department of Oncology, Fuyang Hospital of Anhui Medical University, Fuyang, 236000, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University), No 81 Meishan Road, Hefei 230032, Anhui, China. Electronic address: zhongfei@ahmu.edu.cn.
Biochem Pharmacol ; 223: 116193, 2024 May.
Article en En | MEDLINE | ID: mdl-38582268
ABSTRACT
Ovarian aging leads to infertility and birth defects. We aimed to clarify the role of Indole-3-carbinol (I3C) in resistance to oxidative stress, apoptosis, and fibrosis in ovarian aging. I3C was administered via intraperitoneal injection for 3 weeks in young or old mice. Immunohistochemistry; Masson, Sirius red, and TUNEL staining; follicle counting; estrous cycle analysis; and Western blotting were used for validating the protective effect of I3C against ovarian senescence. Human granulosa-like tumor cell line and primary granulosa cells were used for in vitro assay. The results indicated that I3C inhibited ovarian fibrosis and apoptosis while increasing the number of primordial follicles. Mechanistic studies have shown that I3C promoted the nuclear translocation of nuclear factor-erythroid 2-related factor (Nrf2) and upregulated the expression of heme oxygenase 1 (HO-1). Additionally, I3C increased cell viability and decreased lactate dehydrogenase, malondialdehyde, reactive oxygen species and JC-1 levels. Furthermore, the antioxidant effect of I3C was found to be dependent on the activation of Nrf2 and HO-1, as demonstrated by the disappearance of the effect upon inhibition of Nrf2 expression. In conclusion, I3C can alleviate the ovarian damage caused by aging and may be a protective agent to delay ovarian aging.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hemo-Oxigenasa 1 / Factor 2 Relacionado con NF-E2 / Indoles Límite: Animals / Female / Humans Idioma: En Revista: Biochem Pharmacol Año: 2024 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hemo-Oxigenasa 1 / Factor 2 Relacionado con NF-E2 / Indoles Límite: Animals / Female / Humans Idioma: En Revista: Biochem Pharmacol Año: 2024 Tipo del documento: Article País de afiliación: China