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Virulence Genes and Biofilm Formation Among Legionella pneumophila Isolates Collected from Hospital Water Sources.
Hayatimehr, Sara; Mirkalantari, Shiva; Amirmozafari, Nour; Jazi, Faramarz Masjedian; Moghadam, Majid Taati.
Afiliación
  • Hayatimehr S; Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
  • Mirkalantari S; Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. Mirkalantari.sh@iums.ac.ir.
  • Amirmozafari N; Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
  • Jazi FM; Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
  • Moghadam MT; Department of Microbiology, Guilan University of Medical Sciences, Rasht, Iran.
Curr Microbiol ; 81(6): 141, 2024 Apr 16.
Article en En | MEDLINE | ID: mdl-38625380
ABSTRACT
Legionella pneumophila can be transmitted to people, especially immunocompromised patients, via hospital water pipe systems and cause severe pneumonia. The aim of our study was to investigate the presence of major virulence factor genes, ability of biofilms formation, and correlation between presence of Legionella isolates and temperature, pH, and residual chlorine of water. Hundred water samples were collected from nine hospitals in Tehran, Iran. Temperature, pH, and residual chlorine were determined during sampling. Different virulence genes and the ability to form biofilms were subsequently analyzed among the L. pneumophila isolates. Results showed that 12 (12%) samples were positive in culture method and all of the isolates were positive as L. pneumophila species (mip). A correlation was found between Legionella culture positivity and temperature and pH of water, but there was no significant correlation between residual chlorine of water samples and the presence of Legionella. The isolation of Legionella rate in summer and spring was higher than winter and autumn. Twelve (100%) isolates were positive for mip genes, 9 (75%) for dot genes, 8 (66.66%) for hsp, 6 (50%) for lvh, and 4 (33.33%) for rtx. All of the isolates displayed strong ability for biofilm production every three days. Two of these isolates (16.6%) displayed weak ability to form biofilm on the first day of incubation. This study revealed that water sources in hospitals were colonized by virulent Legionella and should be continuously monitored to avoid elevated concentrations of Legionella with visible biofilm formation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Legionella / Legionella pneumophila Límite: Humans País/Región como asunto: Asia Idioma: En Revista: Curr Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Legionella / Legionella pneumophila Límite: Humans País/Región como asunto: Asia Idioma: En Revista: Curr Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Estados Unidos