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Depolymerization of SUMO chains induces slender to stumpy differentiation in T. brucei bloodstream parasites.
Iribarren, Paula Ana; Di Marzio, Lucía Ayelén; Berazategui, María Agustina; Saura, Andreu; Coria, Lorena; Cassataro, Juliana; Rojas, Federico; Navarro, Miguel; Alvarez, Vanina Eder.
Afiliación
  • Iribarren PA; Instituto de Investigaciones Biotecnológicas "Dr. Rodolfo Ugalde"-IIBIO (UNSAM-CONICET), San Martin, Buenos Aires, Argentina.
  • Di Marzio LA; Instituto de Investigaciones Biotecnológicas "Dr. Rodolfo Ugalde"-IIBIO (UNSAM-CONICET), San Martin, Buenos Aires, Argentina.
  • Berazategui MA; Instituto de Investigaciones Biotecnológicas "Dr. Rodolfo Ugalde"-IIBIO (UNSAM-CONICET), San Martin, Buenos Aires, Argentina.
  • Saura A; Instituto de Parasitología y Biomedicina "López-Neyra", CSIC (IPBLN-CSIC), Granada, Spain.
  • Coria L; Instituto de Investigaciones Biotecnológicas "Dr. Rodolfo Ugalde"-IIBIO (UNSAM-CONICET), San Martin, Buenos Aires, Argentina.
  • Cassataro J; Instituto de Investigaciones Biotecnológicas "Dr. Rodolfo Ugalde"-IIBIO (UNSAM-CONICET), San Martin, Buenos Aires, Argentina.
  • Rojas F; Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, United Kingdom.
  • Navarro M; Instituto de Parasitología y Biomedicina "López-Neyra", CSIC (IPBLN-CSIC), Granada, Spain.
  • Alvarez VE; Instituto de Investigaciones Biotecnológicas "Dr. Rodolfo Ugalde"-IIBIO (UNSAM-CONICET), San Martin, Buenos Aires, Argentina.
PLoS Pathog ; 20(4): e1012166, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38635823
ABSTRACT
Trypanosoma brucei are protozoan parasites that cause sleeping sickness in humans and nagana in cattle. Inside the mammalian host, a quorum sensing-like mechanism coordinates its differentiation from a slender replicative form into a quiescent stumpy form, limiting growth and activating metabolic pathways that are beneficial to the parasite in the insect host. The post-translational modification of proteins with the Small Ubiquitin-like MOdifier (SUMO) enables dynamic regulation of cellular metabolism. SUMO can be conjugated to its targets as a monomer but can also form oligomeric chains. Here, we have investigated the role of SUMO chains in T. brucei by abolishing the ability of SUMO to polymerize. We have found that parasites able to conjugate only SUMO monomers are primed for differentiation. This was demonstrated for monomorphic lines that are normally unable to produce stumpy forms in response to quorum sensing signaling in mice, and also for pleomorphic cell lines in which stumpy cells were observed at unusually low parasitemia levels. SUMO chain mutants showed a stumpy compatible transcriptional profile and better competence to differentiate into procyclics. Our study indicates that SUMO depolymerization may represent a coordinated signal triggered during stumpy activation program.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trypanosoma brucei brucei Límite: Animals / Humans Idioma: En Revista: PLoS Pathog Año: 2024 Tipo del documento: Article País de afiliación: Argentina Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trypanosoma brucei brucei Límite: Animals / Humans Idioma: En Revista: PLoS Pathog Año: 2024 Tipo del documento: Article País de afiliación: Argentina Pais de publicación: Estados Unidos