Suppressive Role of Pigment Epithelium-derived Factor in a Rat Model of Corneal Allograft Rejection.
Transplantation
; 108(10): 2072-2083, 2024 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-38644534
ABSTRACT
BACKGROUND:
Immunological rejection is the most common reason for corneal transplantation failure. The importance of T cells in corneal allograft rejection is well demonstrated. Recent studies highlight that pigment epithelium-derived factor (PEDF) plays an immunoregulatory role in ocular diseases by enhancing the suppressive phenotype of regulatory T cells besides its other functions in neurotrophy and antiangiogenesis.METHODS:
The effects of PEDF on immune rejection were examined in rat models of corneal transplantation using slit-lamp microscope observation, immunohistochemistry, flow cytometry, and Western blot. In vitro, we demonstrated PEDF reduced alloreactive T-cell activation using real-time polymerase chain reaction, flow cytometry, and Western blot.RESULTS:
Topical administration of PEDF provided corneal transplantation rats with an improved graft survival rate of corneal allografts, reduced hemangiogenesis, and infiltration of immune cells in corneas, in particular, type 17 T helper cells while increased regulatory T cells. Moreover, nerve reinnervation within grafts was promoted in PEDF-treated recipient rats. In vitro, PEDF inhibited alloreactive T-cell activation via the c-Jun N-terminal kinase/c-Jun signaling pathway and upregulated the expressions of interleukin-10 and transforming growth factor-ß, emphasizing the suppressive role of PEDF on immune responses.CONCLUSIONS:
Our results underscore the feasibility of PEDF in alleviating corneal allograft rejection and further illustrate its potential in managing immune-related diseases.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ratas Endogámicas Lew
/
Serpinas
/
Trasplante de Córnea
/
Modelos Animales de Enfermedad
/
Proteínas del Ojo
/
Aloinjertos
/
Rechazo de Injerto
/
Supervivencia de Injerto
/
Factores de Crecimiento Nervioso
Límite:
Animals
Idioma:
En
Revista:
Transplantation
Año:
2024
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Estados Unidos