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Ultrasonic-assisted extraction and UHPLC determination of ascorbic acid, polyphenols, and half-maximum effective concentration in Citrus medica and Ziziphus spina-christi fruits using multivariate experimental design.
Belayneh Asfaw, Tilahun; Getachew Tadesse, Mesfin; Beshah Tessema, Fekade; Woldemichael Woldemariam, Henock; V Chinchkar, Ajay; Singh, Anurag; Upadhyay, Ashutosh; Mehari, Bewketu.
Afiliación
  • Belayneh Asfaw T; Department of Chemistry, College of Natural and Computational Sciences, University of Gondar, P.O.Box 196, Gondar, Ethiopia.
  • Getachew Tadesse M; Department of Industrial Chemistry, College of Applied Sciences, Addis Ababa Science and Technology University, P.O.Box 16417, Addis Ababa, Ethiopia.
  • Beshah Tessema F; Center of Excellence for Biotechnology and Bioprocess, Addis Ababa Science and Technology University, P.O.Box 16417, Addis Ababa, Ethiopia.
  • Woldemichael Woldemariam H; Department of Chemistry, College of Natural and Computational Sciences, Woldia University, Woldia, Ethiopia.
  • V Chinchkar A; Department of Chemical Engineering, College of Engineering, Addis Ababa Science and Technology University, P.O.Box 16417, Addis Ababa, Ethiopia.
  • Singh A; Center of Excellence for Biotechnology and Bioprocess, Addis Ababa Science and Technology University, P.O.Box 16417, Addis Ababa, Ethiopia.
  • Upadhyay A; National Institute of Food Technology Entrepreneurship and Management, Department of Food Science and Technology, Haryana, India.
  • Mehari B; National Institute of Food Technology Entrepreneurship and Management, Department of Food Science and Technology, Haryana, India.
Food Chem X ; 22: 101310, 2024 Jun 30.
Article en En | MEDLINE | ID: mdl-38645936
ABSTRACT
This study aimed to determine the concentrations of ascorbic acid and polyphenols in fruits and peels of Citrus medica and Ziziphus spina-christi grown in Ethiopia. Conditions of ultrasound-assisted extraction (UAE) and ultra-high-performance liquid chromatography coupled with a diode array detector (HPLC-DAD) were optimized, using a multivariate experimental design. The optimum conditions of UAE were 15 min extraction time at 35 ℃, with 75 % aqueous methanol as solvent, and a fruit powder-to-solvent ratio (m/v) of 115. Among the different drying conditions investigated, freeze-drying was found to be appropriate for analyzing ascorbic acid, polyphenols, and antioxidant potential. The overall ranges, across the fruits and peels, of ascorbic acid, total polyphenols, and antioxidant potentials (EC50) obtained were 8.7 ± 1.4-91.2 ± 2.6 mg/100 g, 253.0 ± 6.3-764.1 ± 25.8 mg GAE/100 g and 2.4 ± 0.1-26.1 ± 2.9 mg/mL, respectively. This indicates that the fruits and peels of the studied plants are advantageous as sources of ascorbic acid and polyphenols.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Food Chem X Año: 2024 Tipo del documento: Article País de afiliación: Etiopia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Food Chem X Año: 2024 Tipo del documento: Article País de afiliación: Etiopia