Your browser doesn't support javascript.
loading
Rapid autopsies to enhance metastatic research: the UPTIDER post-mortem tissue donation program.
Geukens, Tatjana; De Schepper, Maxim; Van Den Bogaert, Wouter; Van Baelen, Karen; Maetens, Marion; Pabba, Anirudh; Mahdami, Amena; Leduc, Sophia; Isnaldi, Edoardo; Nguyen, Ha-Linh; Bachir, Imane; Hajipirloo, Maysam; Zels, Gitte; Van Cauwenberge, Josephine; Borremans, Kristien; Vandecaveye, Vincent; Weynand, Birgit; Vermeulen, Peter; Leucci, Eleonora; Baietti, Maria Francesca; Sflomos, George; Battista, Laura; Brisken, Cathrin; Derksen, Patrick W B; Koorman, Thijs; Visser, Daan; Scheele, Colinda L G J; Thommen, Daniela S; Hatse, Sigrid; Fendt, Sarah-Maria; Vanderheyden, Evy; Van Brussel, Thomas; Schepers, Rogier; Boeckx, Bram; Lambrechts, Diether; Marano, Giuseppe; Biganzoli, Elia; Smeets, Ann; Nevelsteen, Ines; Punie, Kevin; Neven, Patrick; Wildiers, Hans; Richard, François; Floris, Giuseppe; Desmedt, Christine.
Afiliación
  • Geukens T; Laboratory for Translational Breast Cancer Research, Department of Oncology, KU Leuven, Leuven, Belgium.
  • De Schepper M; Department of General Medical Oncology, University Hospitals Leuven, Leuven, Belgium.
  • Van Den Bogaert W; Laboratory for Translational Breast Cancer Research, Department of Oncology, KU Leuven, Leuven, Belgium.
  • Van Baelen K; Department of Pathology, University Hospitals Leuven, Leuven, Belgium.
  • Maetens M; Department of Forensic Medicine, University Hospitals Leuven, Leuven, Belgium.
  • Pabba A; Laboratory for Translational Breast Cancer Research, Department of Oncology, KU Leuven, Leuven, Belgium.
  • Mahdami A; Department of Gynecology and Obstetrics, University Hospitals Leuven, Leuven, Belgium.
  • Leduc S; Laboratory for Translational Breast Cancer Research, Department of Oncology, KU Leuven, Leuven, Belgium.
  • Isnaldi E; Laboratory for Translational Breast Cancer Research, Department of Oncology, KU Leuven, Leuven, Belgium.
  • Nguyen HL; Laboratory for Translational Breast Cancer Research, Department of Oncology, KU Leuven, Leuven, Belgium.
  • Bachir I; Laboratory for Translational Breast Cancer Research, Department of Oncology, KU Leuven, Leuven, Belgium.
  • Hajipirloo M; Laboratory for Translational Breast Cancer Research, Department of Oncology, KU Leuven, Leuven, Belgium.
  • Zels G; Laboratory for Translational Breast Cancer Research, Department of Oncology, KU Leuven, Leuven, Belgium.
  • Van Cauwenberge J; Laboratory for Translational Breast Cancer Research, Department of Oncology, KU Leuven, Leuven, Belgium.
  • Borremans K; Department of Anesthesiology, Institut Jules Bordet, Brussels, Belgium.
  • Vandecaveye V; Laboratory for Translational Breast Cancer Research, Department of Oncology, KU Leuven, Leuven, Belgium.
  • Weynand B; Laboratory for Translational Breast Cancer Research, Department of Oncology, KU Leuven, Leuven, Belgium.
  • Vermeulen P; Department of Pathology, University Hospitals Leuven, Leuven, Belgium.
  • Leucci E; Laboratory for Translational Breast Cancer Research, Department of Oncology, KU Leuven, Leuven, Belgium.
  • Baietti MF; Department of Gynecology and Obstetrics, University Hospitals Leuven, Leuven, Belgium.
  • Sflomos G; Laboratory for Translational Breast Cancer Research, Department of Oncology, KU Leuven, Leuven, Belgium.
  • Battista L; Department of Gynecology and Obstetrics, University Hospitals Leuven, Leuven, Belgium.
  • Brisken C; Department of Radiology, University Hospitals Leuven, Leuven, Belgium.
  • Derksen PWB; Department of Pathology, University Hospitals Leuven, Leuven, Belgium.
  • Koorman T; Centre for Oncological Research (CORE), University of Antwerp, Antwerp, Belgium.
  • Visser D; TRACE and Laboratory for RNA Cancer Biology, Department of Oncology, KU Leuven, Leuven, Belgium.
  • Scheele CLGJ; TRACE and Laboratory for RNA Cancer Biology, Department of Oncology, KU Leuven, Leuven, Belgium.
  • Thommen DS; ISREC - Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
  • Hatse S; ISREC - Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
  • Fendt SM; ISREC - Swiss Institute for Experimental Cancer Research, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.
  • Vanderheyden E; The Breast Cancer Now Toby Robins Breast Cancer Research Centre, The Institute of Cancer Research, London, UK.
  • Van Brussel T; Department of Pathology, University Medical Center, Utrecht, The Netherlands.
  • Schepers R; Department of Pathology, University Medical Center, Utrecht, The Netherlands.
  • Boeckx B; Department of Pathology, University Medical Center, Utrecht, The Netherlands.
  • Lambrechts D; Laboratory of Intravital Microscopy and Dynamics of Tumor Progression, Department of Oncology, VIB-KU Leuven Center for Cancer Biology, Leuven, Belgium.
  • Marano G; Division of Molecular Oncology and Immunology, Netherlands Cancer Institute, Amsterdam, The Netherlands.
  • Biganzoli E; Laboratory of Experimental Oncology, Department of Oncology, KU Leuven, Leuven, Belgium.
  • Smeets A; Laboratory of Cellular Metabolism and Metabolic Regulation, VIB-KU Leuven Center for Cancer Biology, VIB, Leuven, Belgium.
  • Nevelsteen I; Laboratory of Cellular Metabolism and Metabolic Regulation, Department of Oncology, KU Leuven and Leuven Cancer Institute (LKI), Leuven, Belgium.
  • Punie K; Laboratory for Translational Genetics, Department of Human Genetics, KU Leuven, Leuven, Belgium, and VIB Center for Cancer Biology, Leuven, Belgium.
  • Neven P; Laboratory for Translational Genetics, Department of Human Genetics, KU Leuven, Leuven, Belgium, and VIB Center for Cancer Biology, Leuven, Belgium.
  • Wildiers H; Laboratory for Translational Genetics, Department of Human Genetics, KU Leuven, Leuven, Belgium, and VIB Center for Cancer Biology, Leuven, Belgium.
  • Richard F; Laboratory for Translational Genetics, Department of Human Genetics, KU Leuven, Leuven, Belgium, and VIB Center for Cancer Biology, Leuven, Belgium.
  • Floris G; Laboratory for Translational Genetics, Department of Human Genetics, KU Leuven, Leuven, Belgium, and VIB Center for Cancer Biology, Leuven, Belgium.
  • Desmedt C; Unit of Medical Statistics, Biometry and Epidemiology, Department of Biomedical and Clinical Sciences (DIBIC) "L. Sacco" & DSRC, LITA Vialba campus, Università degli Studi di Milano, Milan, Italy.
NPJ Breast Cancer ; 10(1): 31, 2024 Apr 24.
Article en En | MEDLINE | ID: mdl-38658604
ABSTRACT
Research on metastatic cancer has been hampered by limited sample availability. Here we present the breast cancer post-mortem tissue donation program UPTIDER and show how it enabled sampling of a median of 31 (range 5-90) metastases and 5-8 liquids per patient from its first 20 patients. In a dedicated experiment, we show the mild impact of increasing time after death on RNA quality, transcriptional profiles and immunohistochemical staining in tumor tissue samples. We show that this impact can be counteracted by organ cooling. We successfully generated ex vivo models from tissue and liquid biopsies from distinct histological subtypes of breast cancer. We anticipate these and future findings of UPTIDER to elucidate mechanisms of disease progression and treatment resistance and to provide tools for the exploration of precision medicine strategies in the metastatic setting.
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: NPJ Breast Cancer Año: 2024 Tipo del documento: Article País de afiliación: Bélgica
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: NPJ Breast Cancer Año: 2024 Tipo del documento: Article País de afiliación: Bélgica