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SARS-CoV-2 live virus culture and sample freeze-thaw stability.
Kanki, Phyllis J; Hamel, Donald J; Riedel, Stefan; Dutta, Sanjucta; Cheng, Annie; Chang, Charlotte A; Arnaout, Ramy; Kirby, James E.
Afiliación
  • Kanki PJ; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, 651 Huntington Avenue, Boston, MA 02115, USA. Electronic address: pkanki@hsph.harvard.edu.
  • Hamel DJ; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, 651 Huntington Avenue, Boston, MA 02115, USA.
  • Riedel S; Department of Pathology, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA; Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, USA.
  • Dutta S; Department of Pathology, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA.
  • Cheng A; Department of Pathology, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA.
  • Chang CA; Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, 651 Huntington Avenue, Boston, MA 02115, USA.
  • Arnaout R; Department of Pathology, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA; Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, USA; Division of Clinical Informatics, Department of Medicine, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston,
  • Kirby JE; Department of Pathology, Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA; Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, USA.
Diagn Microbiol Infect Dis ; 109(3): 116282, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38663335
ABSTRACT
The effect of freeze-thaw on SARS-CoV-2 viral viability is not well established. We isolated virus from 31 split clinical samples cultured fresh or after a 7- or 17/18-day freeze. We found that freeze-thaw did not significantly affect viral culture isolation. Therefore, frozen samples may be used to assess SARS-CoV-2 infectiousness.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Congelación / SARS-CoV-2 / COVID-19 Límite: Humans Idioma: En Revista: Diagn Microbiol Infect Dis Año: 2024 Tipo del documento: Article
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Congelación / SARS-CoV-2 / COVID-19 Límite: Humans Idioma: En Revista: Diagn Microbiol Infect Dis Año: 2024 Tipo del documento: Article