Feasibility of Direct Vitrectomy-Sparing Subretinal Injection for Gene Delivery in Large Animals.
Curr Eye Res
; 49(8): 879-887, 2024 Aug.
Article
en En
| MEDLINE
| ID: mdl-38666493
ABSTRACT
PURPOSE:
To assess the safety and feasibility of direct vitrectomy-sparing subretinal injection for gene delivery in a large animal model.METHODS:
The experimental Libechov minipigs were used for subretinal delivery of a plasmid DNA vector (pS/MAR-CMV-copGFP) with cytomegalovirus (CMV) promoter, green fluorescent protein (GFP) reporter (copGFP) and a scaffold/matrix attachment region (S/MAR) sequence. The eyes were randomized to subretinal injection of the vector following pars plana vitrectomy (control group) or a direct injection without prior vitrectomy surgery (experimental group). Intra- and post-operative observations up to 30 days after surgery were compared.RESULTS:
Six eyes of three mini-pigs underwent surgery for delivery into the subretinal space. Two eyes in the control group were operated with a classical approach (lens-sparing vitrectomy and posterior hyaloid detachment). The other four eyes in the experimental group were injected directly with a subretinal cannula without vitrectomy surgery. No adverse events, such as endophthalmitis, retinal detachment and intraocular pressure elevation were observed post-operatively. The eyes in the experimental group had both shorter surgical time and recovery while achieving the same surgical goal.CONCLUSIONS:
This pilot study demonstrates that successful subretinal delivery of gene therapy vectors is achievable using a direct injection without prior vitrectomy surgery.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Porcinos Enanos
/
Vitrectomía
/
Terapia Genética
/
Estudios de Factibilidad
/
Técnicas de Transferencia de Gen
/
Vectores Genéticos
Límite:
Animals
Idioma:
En
Revista:
Curr Eye Res
Año:
2024
Tipo del documento:
Article
País de afiliación:
República Checa
Pais de publicación:
Reino Unido