Development of PCR-based genetic test for detection of TTF1 mutation causing abortion in Holstein Friesian cattle.
Reprod Domest Anim
; 59(5): e14581, 2024 May.
Article
en En
| MEDLINE
| ID: mdl-38698693
ABSTRACT
A stop-gain mutation (rs715966442; BTA11 1,02,463,944 nucleotide position) in transcription termination factor, RNA polymerase I (TTF1) gene causes abortion in Holstein Friesian (HF) cattle. A PCR-restriction fragment length polymorphism (PCR-RFLP)-based genetic test has been developed and validated to screen the TTF1 mutation locus in HF cattle. The mutation locus was screened in 80 HF and HF crossbreds using the protocol, which revealed two animals as carriers of the mutant TTF1 allele. The test employed is cost-effective, rapid and precise and can be utilized as an effective tool for the screening of TTF1 mutation carriers in HF cattle population.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polimorfismo de Longitud del Fragmento de Restricción
/
Enfermedades de los Bovinos
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Reacción en Cadena de la Polimerasa
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Aborto Veterinario
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Mutación
Límite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Reprod Domest Anim
Asunto de la revista:
MEDICINA REPRODUTIVA
/
MEDICINA VETERINARIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
India