Two populations of protein molecules detected by small-angle neutron and X-ray scattering (SANS and SAXS) in lyophilized protein:lyoprotector (disaccharide) systems.
Soft Matter
; 20(19): 3897-3900, 2024 May 15.
Article
en En
| MEDLINE
| ID: mdl-38700293
ABSTRACT
Two protein interaction peaks are observed in pharmaceutically-relevant protein (serum albumin) disaccharide 1 1 and 1 3 (w/w) freeze-dried systems for the first time. In samples with a higher disaccharide content, the protein-protein distances are longer for both populations, while the fraction of the protein population with a shorter protein-protein distance is lower. Both factors would favor better stability against aggregation for disaccharide-rich protein formulations. This study provides direct experimental support for a "dilution" hypothesis as a potential stabilization mechanism for freeze-dried protein formulations.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Difracción de Rayos X
/
Disacáridos
/
Dispersión del Ángulo Pequeño
/
Liofilización
Límite:
Animals
Idioma:
En
Revista:
Soft Matter
Año:
2024
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Reino Unido