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Characterization of Shiga Toxin-producing Escherichia coli Isolated from Cattle Around Ulaanbaatar City, Mongolia.
Bulgan, Erdenebat; Byambajav, Zolzaya; Ayushjav, Narantuya; Hirai, Yuji; Tanaka, Misaki; Purevdorj, Nyam-Osor; Badrakh, Sandagdorj; Suzuki, Akio; Komatsu, Yusuke; Sato, Toyotaka; Horiuchi, Motohiro.
Afiliación
  • Bulgan E; Laboratory of Veterinary Hygiene, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo 060-0818, Japan.
  • Byambajav Z; Laboratory of Veterinary Sanitation and Hygiene, Ulaanbaatar Veterinary Department, Chinguunjav Street, 2nd Khoroo, Bayangol District, Ulaanbaatar 16050, Mongolia.
  • Ayushjav N; Laboratory of Veterinary Sanitation and Hygiene, Ulaanbaatar Veterinary Department, Chinguunjav Street, 2nd Khoroo, Bayangol District, Ulaanbaatar 16050, Mongolia.
  • Hirai Y; Laboratory of Veterinary Hygiene, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo 060-0818, Japan.
  • Tanaka M; Laboratory of Veterinary Hygiene, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo 060-0818, Japan.
  • Purevdorj NO; School of Veterinary Medicine, Mongolian University of Life Science, Zaisan, Khan-Uul, Ulaanbaatar 17024, Mongolia.
  • Badrakh S; School of Veterinary Medicine, Mongolian University of Life Science, Zaisan, Khan-Uul, Ulaanbaatar 17024, Mongolia.
  • Suzuki A; Laboratory of Veterinary Hygiene, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo 060-0818, Japan; One Health Research Center, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo 060-0818, Japan.
  • Komatsu Y; Laboratory of Veterinary Hygiene, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo 060-0818, Japan.
  • Sato T; Laboratory of Veterinary Hygiene, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo 060-0818, Japan; One Health Research Center, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo 060-0818, Japan.
  • Horiuchi M; Laboratory of Veterinary Hygiene, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo 060-0818, Japan; One Health Research Center, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo 060-0818, Japan. Electronic address:
J Food Prot ; 87(7): 100294, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38718985
ABSTRACT
Shiga toxin-producing Escherichia coli (STEC) are associated with severe infections including hemorrhagic colitis and hemolytic uremic syndrome in humans. Ruminants are known as reservoirs of STEC; however, no data are available on STEC in ruminants in Mongolia, where more than 5 million cattle and 25 million sheep are raised. To disclose the existence and characteristics of STEC in Mongolia, in this study, we isolated and characterized STEC from cattle in Mongolia. We collected 350 rectal swabs of cattle from 30 farms near Ulaanbaatar city and isolated 45 STEC from 21 farms. Rectal swabs were precultured with modified Escherichia coli broth and then inoculated to Cefixime-Tellurite Sorbitol MacConkey agar plate and/or CHROMagar STEC agar plate for the isolation of STEC. The isolation ratios in each farm were from 0% to 40%. Multiplex PCR for the estimation of O- and H-serotypes identified 12 O-genotypes (Og-types) and 11 H-genotypes (Hg-types) from 45 isolates; however, Og-types of 19 isolates could not be determined. Stx gene subtyping by PCR identified 2 stx1 subtypes (1a and 1c) and 4 stx2 subtypes (2a, 2c, 2d, and 2g). Forty-five isolates were divided into 21 different groups based on the Og- and Hg-types, stx gene subtypes and the existence of virulence factors, ehxA, eae, and saa, which includes several major serotypes associated with human illness such as O26H11 and O157H7. The most dominant isolate, OgUTH19 [stx1a (+), stx2a (+), ehxA (+) and saa (+)], was isolated from eight farms. This is the first report on the characterization of STEC in cattle in Mongolia, and the results suggest the importance of further monitoring of STEC contamination in the food chains as well as STEC infection in humans.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Escherichia coli / Escherichia coli Shiga-Toxigénica Límite: Animals / Humans País/Región como asunto: Asia Idioma: En Revista: J Food Prot Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Escherichia coli / Escherichia coli Shiga-Toxigénica Límite: Animals / Humans País/Región como asunto: Asia Idioma: En Revista: J Food Prot Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos