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Molecular epidemiology of human herpesvirus 8 in patients with HHV-8-related diseases in Ireland.
O'Rourke, Sadhbh; Laoi, Bairbre Ni; Clarke, Susan; Crowley, Brendan.
Afiliación
  • O'Rourke S; Department of Clinical Microbiology, St. James's Hospital, Dublin, Ireland.
  • Laoi BN; Department of Clinical Microbiology, St. James's Hospital, Dublin, Ireland.
  • Clarke S; Department of Clinical Virology, St. James's Hospital, Dublin, Ireland.
  • Crowley B; Department of Genitourinary Medicine and Infectious Diseases, St. James's Hospital, Dublin, Ireland.
J Med Virol ; 96(5): e29654, 2024 May.
Article en En | MEDLINE | ID: mdl-38727099
ABSTRACT
Human Herpesvirus 8 (HHV-8) has been classified by sequence analysis of open reading frame (ORF) K1, ORF K15, and variable sequence loci within the central constant region. The purpose of this study was to examine the molecular epidemiology of HHV-8 in an Irish population. This retrospective study included 30 patients who had HHV-8 DNA detected in plasma. Nested end-point PCR was used to characterise four regions of the HHV-8 genome, K1, T0.7 (K12), ORF 75, and K15. Sequencing data were obtained for 23 specimens from 19 patients. Phylogenetic analysis of ORF K1 demonstrated that subtypes A, B, C and F were present in 37%, 11%, 47% and 5%, respectively. For T0.7 and ORF 75, sequencing data were obtained for 12 patients. For T0.7, subtypes A/C, J, B, R and Q were present in 58%, 17%, 8%, 8%, and 8%, respectively. For ORF 75, subtypes A, B, C and D were present in 58%, 8%, 25%, and 8%, respectively. K15 sequences were determined for 13 patients. 69% had the P allele and 31% had the M allele. The data generated by this study demonstrate that a broad variety of HHV-8 subtypes are represented in patients exhibiting HHV-8-related disease in Ireland, a low prevalence country. The predominance of C and A K1 subtypes was as expected for a Western European population. The 31% prevalence for K15 subtype M was higher than expected for a Western European population. This may represent the changing and evolving epidemiology in Ireland due to altered migration patterns.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / ADN Viral / Análisis de Secuencia de ADN / Epidemiología Molecular / Infecciones por Herpesviridae / Herpesvirus Humano 8 Límite: Adolescent / Adult / Aged / Aged80 / Child / Female / Humans / Male / Middle aged País/Región como asunto: Europa Idioma: En Revista: J Med Virol Año: 2024 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / ADN Viral / Análisis de Secuencia de ADN / Epidemiología Molecular / Infecciones por Herpesviridae / Herpesvirus Humano 8 Límite: Adolescent / Adult / Aged / Aged80 / Child / Female / Humans / Male / Middle aged País/Región como asunto: Europa Idioma: En Revista: J Med Virol Año: 2024 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Estados Unidos