A novel NIR fluorescent probe for enhanced ß-galactosidase detection and tumor imaging in ovarian cancer models.
Spectrochim Acta A Mol Biomol Spectrosc
; 317: 124411, 2024 Sep 05.
Article
en En
| MEDLINE
| ID: mdl-38728851
ABSTRACT
The advancement of biological imaging techniques critically depends on the development of novel near-infrared (NIR) fluorescent probes. In this study, we introduce a designed NIR fluorescent probe, NRO-ßgal, which exhibits a unique off-on response mechanism to ß-galactosidase (ß-gal). Emitting a fluorescence peak at a wavelength of 670 nm, NRO-ßgal showcases a significant Stokes shift of 85 nm, which is indicative of its efficient energy transfer and minimized background interference. The probe achieves a remarkably low in vitro detection limit of 0.2 U/L and demonstrates a rapid response within 10 min, thereby underscoring its exceptional sensitivity, selectivity, and operational swiftness. Such superior analytical performance broadens the horizon for its application in intricate biological imaging studies. To validate the practical utility of NRO-ßgal in bio-imaging, we employed ovarian cancer cell and mouse models, where the probe's efficacy in accurately delineating tumor cells was examined. The results affirm NRO-ßgal's capability to provide sharp, high-contrast images of tumor regions, thereby significantly enhancing the precision of surgical tumor resection. Furthermore, the probe's potential for real-time monitoring of enzymatic activity in living tissues underscores its utility as a powerful tool for diagnostics in oncology and beyond.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Ováricas
/
Beta-Galactosidasa
/
Colorantes Fluorescentes
Límite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Spectrochim Acta A Mol Biomol Spectrosc
/
Spectrochim. acta, Part A, Mol. biomol. spectrosc. (Print)
/
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (Print)
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2024
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Reino Unido