Rapid and practical colorimetric biosensor for leishmaniasis diseases.
Diagn Microbiol Infect Dis
; 109(4): 116352, 2024 Aug.
Article
en En
| MEDLINE
| ID: mdl-38768547
ABSTRACT
In this article, a colorimetric biosensor for detection of Leishmania major surface protease (Gp63) antibody (anti-gp63) was developed by using gold nanoparticle (AuNP) as a color reagent. The dispersion or aggregation of AuNPs leads to a distinct and sensitive change in UV-vis spectra and solution color. For this purpose, kinetoplastid membrane protein-11 (KMP-11) was labeled with AuNPs surface directly. After that, Gp63 antibody was added in the KMP-11@AuNP solution and a color change from red/pink to purple/violet was observed. As a result, anti-gp63 solution diluted at a ratio of 1640 can be detected with the developed colorimetric leishmania biosensor. The relative standard deviation value for 1320 diluted anti-gp63 was calculated as 1.29 %. Furthermore, the linear range of the developed colorimetric biosensor was determined as 180 to 1640. Moreover, developed Leishmania biosensor was applied for detection of leishmania parasite crude antigen and rabbit serum which were used as positive and negative samples respectively. As a result, the recovery values for the measurements of aforementioned samples were calculated as 95.3 % ± 0.02, 103.1 % ± 0.02, 96.2 % ± 0.01 and 95.5 % ± 0.03 for dilutions of 1200, 1160, 1320 and 1640 anti-gp63 solutions respectively.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Leishmaniasis
/
Colorimetría
/
Nanopartículas del Metal
/
Oro
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
/
Diagn. microbiol. infect. dis
/
Diagnostic microbiology and infectious disease
Año:
2024
Tipo del documento:
Article
País de afiliación:
Turquía
Pais de publicación:
Estados Unidos