In-cell chemical construction of a photoswitchable CENP-E using a photochromic covalent inhibitor.

Matsuo, Kazuya; Yamaoka, Shusuke; Waku, Tomonori; Kobori, Akio

Matsuo, Kazuya; Yamaoka, Shusuke; Waku, Tomonori; Kobori, Akio.

Afiliación

Matsuo K; Faculty of Molecular Chemistry and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan. kmatsuo@kit.ac.jp.
Yamaoka S; Faculty of Molecular Chemistry and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan. kmatsuo@kit.ac.jp.
Waku T; Faculty of Molecular Chemistry and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan. kmatsuo@kit.ac.jp.
Kobori A; Faculty of Molecular Chemistry and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan. kmatsuo@kit.ac.jp.

Org Biomol Chem ; 22(23): 4651-4655, 2024 06 12.

Article en En | MEDLINE | ID: mdl-38787760

ABSTRACT

ABSTRACT

An arylazopyrazole-based covalent inhibitor targeting the mitotic motor protein of centromere-associated protein E (CENP-E) was developed. Using this photoswitchable inhibitor, a photoswitchable CENP-E was chemically constructed in cells, which enabled to local control of mitotic cell division with light illumination.

Asunto(s)

Proteínas Cromosómicas no Histona; Humanos; Proteínas Cromosómicas no Histona/antagonistas & inhibidores; Proteínas Cromosómicas no Histona/metabolismo; Procesos Fotoquímicos; Luz; Pirazoles/química; Pirazoles/farmacología; Pirazoles/síntesis química; Células HeLa; Estructura Molecular; Mitosis/efectos de los fármacos

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Cromosómicas no Histona Límite: Humans Idioma: En Revista: Org Biomol Chem Asunto de la revista: BIOQUIMICA / QUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: ENGLAND / ESCOCIA / GB / GREAT BRITAIN / INGLATERRA / REINO UNIDO / SCOTLAND / UK / UNITED KINGDOM

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