Efficacy of RCI001 as a therapeutic candidate of dry eye disease in a modified mixed dry eye model.
Eye Vis (Lond)
; 11(1): 19, 2024 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-38822444
ABSTRACT
BACKGROUND:
To evaluate the therapeutic effects of topical RCI001 (RCI) and compare its efficacy with that of 1% prednisolone acetate (PDE) and 5% Lifitegrast in a modified mixed dry eye disease (DED) model.METHODS:
The environmental DED model was induced in BALB/c mice in a dry chamber with scopolamine. The eyes of the mice were treated topically with phosphate buffered saline (PBS), PDE, Lifitegrast or RCI twice daily for 1 week. Ocular surface staining (OSS), tear secretion, inflammatory cytokines in the ocular surface and lacrimal gland, and immunofluorescence staining in the conjunctiva and cornea(CC) were assessed.RESULTS:
The RCI group demonstrated better improvement of OSS and tear secretion than the PBS group (OSS, PBS 13.0 ± 1.6, RCI 9.4 ± 3.0; tear secretion, PBS 5.0 ± 0.4 mm, RCI 7.0 ± 0.3 mm, each P < 0.001) and better clinical efficacy than PDE and Lifitegrast groups on Day 7 (improvement rate of OSS, RCI 32.45%, Lifitegrast 13.13%, PDE 12.25%). The RCI group resulted in significantly lower expression of oxidative stress markers in the CC than the PBS group (4-HNE, NOX2, and NOX4 in the conjunctiva; NOX2 in the cornea, each P < 0.05). However, the PDE and Lifitegrast groups did not show significant differences compared with the PBS group. There were no significant differences of inflammatory cytokines in the ocular surface and lacrimal gland between all groups.CONCLUSION:
Topical RCI001 showed excellent therapeutic effects in environmental DED models by stimulating tear secretion, modulating oxidative stress and improving corneal epithelial healing compared to 1% PDE and 5% Lifitegrast.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Eye Vis (Lond)
Año:
2024
Tipo del documento:
Article